Analytical Data
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Gene name
SNAP25
- Application
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Alternative Names
SNAP25;SNAP;Synaptosomal-associated Protein 25
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P60880
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Expression Region
1-206aa
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AA Sequence
MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLDRVEEGMNHINQDMKEAEKNLKDLGKCCGLFICPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG
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Molecular Weight
27.3kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SNAP25 (Synaptosomal-associated protein of 25 kDa) is a crucial component of the synaptic vesicle release machinery in neurons, playing an essential role in neurotransmission. It is a member of the SNAP (soluble N-ethylmaleimide-sensitive factor attachment protein) family, which participates in the docking and fusion of synaptic vesicles with the presynaptic membrane. Research into SNAP25 has gained prominence due to its involvement in various neurological disorders, including schizophrenia, epilepsy, and neurodegenerative diseases. The functional properties of SNAP25 are intricately linked to its ability to form complexes with other proteins, such as syntaxins and synaptobrevins, which facilitate the precise exocytosis of neurotransmitters. Recombination technologies, including molecular cloning and protein expression systems, allow for the production of recombinant SNAP25, enabling detailed studies on its structure-function relationship and interaction dynamics. Understanding SNAP25's role at the synapse, its post-translational modifications, and its implications in synaptic plasticity are critical areas of research, offering potential therapeutic targets for modulating synaptic function in various pathologies. Current studies often focus on elucidating how alterations in SNAP25 dynamics can contribute to synaptic dysfunction, underscoring the importance of this protein in both basic neuroscience and the development of innovative treatments for brain disorders.
