Analytical Data
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基因名
RFC3
- Application
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别名
RFC3；Replication factor C subunit 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P40938
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表达区间
1-356aa
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氨基酸序列
MSLWVDKYRP CSLGRLDYHK EQAAQLRNLV QCGDFPHLLV YGPSGAGKKT RIMCILRELY GVGVEKLRIE HQTITTPSKK KIEISTIASN YHLEVNPSDA GNSDRVVIQE MLKTVAQSQQ LETNSQRDFK VVLLTEVDKL TKDAQHALRR TMEKYMSTCR LILCCNSTSK VIPPIRSRCL AVRVPAPSIE DICHVLSTVC KKEGLNLPSQ LAHRLAEKSC RNLRKALLMC EACRVQQYPF TADQEIPETD WEVYLRETAN AIVSQQTPQR LLEVRGRLYE LLTHCIPPEI IMKGLLSELL HNCDGQLKGE VAQMAAYYEH RLQLGSKAIY HLEAFVAKFM ALYKKFMEDG LEGMMF
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分子量
40.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RFC3, or Replication Factor C subunit 3, is a critical component of the replication factor C (RFC) complex, which plays a vital role in DNA replication and repair processes. Research on RFC3 has gained significance due to its involvement in cellular responses to DNA damage and maintenance of genome stability. As a part of the RFC complex, RFC3 is essential for loading the sliding clamp protein PCNA (Proliferating Cell Nuclear Antigen) onto DNA, which is crucial for efficient DNA synthesis and the coordination of DNA repair mechanisms. Dysregulation or mutations in RFC3 have been implicated in various human diseases, including cancer, where altered DNA repair pathways can lead to genomic instability. Understanding the structure and function of RFC3 can provide insights into its role in these critical biological processes and may reveal potential therapeutic targets for diseases associated with defective DNA replication and repair. Consequently, ongoing research is focused on elucidating the molecular mechanisms of RFC3, including its interactions with other proteins and its regulation during the cell cycle, highlighting its importance in maintaining cellular integrity and preventing tumorigenesis.
