Analytical Data
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Gene name
ACCN2
- Application
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Alternative Names
ACCN2; ACCN2_HUMAN; Acid sensing ion channel 1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal.
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P78348
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Expression Region
1-528aa
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AA Sequence
MELKAEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFHYHHVTKLDEVAASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQDKANFRSFKPKPFNMREFYDRAGHDIRDMLLSCHFRGEVCSAEDFKVVFTRYGKCYTFNSGRDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVACQEQRLIYLPPPWGTCKAVTMDSDLDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHKLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC
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Molecular Weight
86.3 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The ACCN2 (acid-sensing ion channel 2) protein is a member of the amiloride-sensitive sodium channel family, playing a crucial role in the detection of changes in extracellular acidity. It is predominantly expressed in the central nervous system and plays a significant role in various physiological processes, including pain sensation, neuroprotection, and modulation of synaptic activity. Research into ACCN2 has gained momentum due to its involvement in pathological conditions, such as chronic pain and neurodegenerative diseases, where altered acid sensing may contribute to neuronal excitability and inflammation. The recombinant expression of ACCN2 has become a pivotal focus for understanding its structure-function relationship and for evaluating its potential as a therapeutic target. Through the production of recombinant ACCN2 proteins, researchers can investigate biophysical properties, ion selectivity, and pharmacological interactions, facilitating the development of drugs to modulate its activity. Furthermore, structural studies of ACCN2 provide insights into the molecular mechanisms underlying its function and regulation. As research progresses, the exploration of ACCN2's role in disease contexts may yield novel avenues for intervention in acid-related pathologies and enhance our understanding of ion channel biology.
