Recombinant Human SIRT1 Protein，His

SIRT1

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

SIRT1;SIR2L1;NAD-dependent Protein deacetylase sirtuin-1

Human

E. coli

His tag N-Terminus

Greater than 90% as determined by SDS-PAGE.

Q96EB6

193-741aa

MIGTDPRTIL KDLLPETIPP PELDDMTLWQ IVINILSEPP KRKKRKDINT IEDAVKLLQE CKKIIVLTGA GVSVSCGIPD FRSRDGIYAR LAVDFPDLPD PQAMFDIEYF RKDPRPFFKF AKEIYPGQFQ PSLCHKFIAL SDKEGKLLRN YTQNIDTLEQ VAGIQRIIQC HGSFATASCL ICKYKVDCEA VRGDIFNQVV PRCPRCPADE PLAIMKPEIV FFGENLPEQF HRAMKYDKDE VDLLIVIGSS LKVRPVALIP SSIPHEVPQI LINREPLPHL HFDVELLGDC DVIINELCHR LGGEYAKLCC NPVKLSEITE KPPRTQKELA YLSELPPTPL HVSEDSSSPE RTSPPDSSVI VTLLDQAAKS NDDLDVSESK GCMEEKPQEV QTSRNVESIA EQMENPDLKN VGSSTGEKNE RTSVAGTVRK CWPNRVAKEQ ISRRLDGNQY LFLPPNRYIF HGAEVYSDSE DDVLSSSSCG SNSDSGTCQS PSLEEPMEDE SEIEEFYNGL EDEPDVPERA GGAGFGTDGD DQEAINEAIS VKQEVTDMN

88 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.