Analytical Data
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Gene name
COL4
- Application
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Alternative Names
COL4；Collagen alpha-1(XIX) chain
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q940T9
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Expression Region
1-362aa
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AA Sequence
MASKLCDSCKSATAALYCRPDAAFLCLSCDSKVHAANKLASRHARVWMCEVCEQAPAHVTCKADAAALCVTCDRDIHSANPLARRHERVPVTPFYDSVSSDGSVKHTAVNFLDDCYFSDIDGNGSREEEEEEAASWLLLPNPKTTTTATAGIVAVTSAEEVPGDSPEMNTGQQYLFSDPDPYLDLDYGNVDPKVESLEQNSSGTDGVVPVENRTVRIPTVNENCFEMDFTGGSKGFTYGGGYNCISHSVSSSSMEVGVVPDGGSVADVSYPYGGPATSGADPGTQRAVPLTSAEREARVMRYREKRKNRKFEKTIRYASRKAYAEMRPRIKGRFAKRTDTNESNDVVGHGGIFSGFGLVPTF
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Molecular Weight
39.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
COL4 (Collagen Type IV) is a crucial component of the basement membrane, playing a vital role in maintaining tissue integrity and function. As a pivotal structural protein, COL4 is composed of various chains that form a unique network, providing support to various organs and influencing cellular behaviors such as adhesion, migration, and differentiation. Research on COL4 has gained prominence due to its implications in various pathological conditions, including kidney diseases, cancer metastasis, and vascular disorders. Mutations or abnormal expression of COL4 can lead to disorders like Alport syndrome, which is characterized by kidney failure, hearing loss, and eye abnormalities. Furthermore, understanding the molecular mechanisms underlying COL4 function and its interactions with other extracellular matrix components can unveil potential therapeutic targets for regeneration and repair. The exploration of COL4 recombinant proteins has opened avenues for innovative research in regenerative medicine and tissue engineering, offering prospects for developing biomaterials that mimic native ECM properties. By studying COL4 recombinants, researchers aim to elucidate its biophysical properties, how it influences cell behavior, and its potential in enhancing wound healing and tissue repair processes. This growing interest underscores the significance of COL4 in both physiological and pathological contexts, paving the way for future discoveries and applications in biomedical research.
