Analytical Data
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Gene name
DOC2b
- Application
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Alternative Names
DOC2b；DOC2BL；Double C2-like domain-containing Protein beta
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14184
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Expression Region
1-412aa
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AA Sequence
MTLRRRGEKA TISIQEHMAI DVCPGPIRPI KQISDYFPRF PRGLPPDAGP RAAAPPDAPA RPAVAGAGRR SPSDGAREDD EDVDQLFGAY GSSPGPSPGP SPARPPAKPP EDEPDADGYE SDDCTALGTL DFSLLYDQEN NALHCTITKA KGLKPMDHNG LADPYVKLHL LPGASKANKL RTKTLRNTLN PTWNETLTYY GITDEDMIRK TLRISVCDED KFRHNEFIGE TRVPLKKLKP NHTKTFNICL EKQLPVDKTE DKSLEERGRI LISLKYSSQK QGLLVGIVRC AHLAAMDANG YSDPYVKTYL RPDVDKKSKH KTAVKKKTLN PEFNEEFCYE IKHGDLAKKS LEVTVWDYDI GKSNDFIGGV VLGIHAKGER LKHWFDCLKN KDKRIERWHT LTSELPGAVL SD
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Molecular Weight
45.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DOC2b, a member of the DOC2 protein family, plays a crucial role in neuronal communication and synaptic function. It is primarily expressed in the brain and is involved in exocytosis and neurotransmitter release. Research on DOC2b has gained momentum due to its potential implications in understanding neurological disorders such as Alzheimer's disease and schizophrenia, where dysregulation of synaptic transmission is frequently observed. Studies have shown that DOC2b interacts with synaptic vesicles and is influenced by calcium ions, suggesting its pivotal role in the calcium-dependent release of neurotransmitters. Additionally, the protein has been implicated in the modulation of synaptic efficacy and plasticity, which are essential for learning and memory processes. Given its importance in neural signaling, the recombinant production of DOC2b has become a focal point of investigation, facilitating in-depth studies of its structure-function relationships and interactions with other synaptic proteins. This recombinant protein research not only enhances the understanding of DOC2b's biological functions but also paves the way for potential therapeutic strategies targeting synaptic dysfunction in various neurological conditions. As researchers aim to elucidate the precise mechanisms by which DOC2b contributes to synaptic regulation, the recombinant protein provides a valuable tool for experimental assays, including binding studies, functional assays, and molecular interaction analyses.
