Analytical Data
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Gene name
NR3C2
- Application
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Alternative Names
Aldosterone receptor; MCR; MCR_HUMAN; MGC133092; Mineralocorticoid receptor; MLR; MR; NR3 C2; NR3C2; NR3C2 protein; Nuclear receptor subfamily 3 group C member 2
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P08235
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Expression Region
1-110 aa
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AA Sequence
METKGYHSLPEGLDMERRWGQVSQAVERSSLGPTERTDENNYMEIVNVSCVSGAIPNNSTQGSSKEKQELLPCLQQDNNRPGILTSDIKTELESKELSATVAESMGLYMD
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Molecular Weight
37.84 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NR3C2, also known as the mineralocorticoid receptor (MR), is a steroid hormone receptor that plays a crucial role in regulating electrolyte balance, blood pressure, and cardiovascular function. Research on NR3C2 has gained significant attention due to its involvement in various physiological and pathophysiological processes, including hypertension, heart failure, and metabolic disorders. The receptor is activated by mineralocorticoids, primarily aldosterone, which exert their effects on gene expression and cellular signaling. Given its critical role in the renin-angiotensin-aldosterone system (RAAS), NR3C2 has become a focal point for understanding the molecular mechanisms underlying cardiovascular diseases. Recombinant NR3C2 proteins have been developed to facilitate studies on its structure, function, and interactions with ligands and other cellular partners. These recombinant proteins enable researchers to dissect the complex signaling pathways mediated by NR3C2 and to explore potential therapeutic targets for conditions associated with dysregulated NR3C2 activity. Additionally, laboratory studies utilizing NR3C2 reconstitution systems are essential for drug development aimed at modulating mineralocorticoid signaling, which could lead to innovative treatments for hypertension and related disorders. Overall, the study of NR3C2 recombinant proteins is a promising avenue for advancing our understanding of the receptor's role in health and disease, paving the way for the development of targeted therapies that could improve patient outcomes in various cardiovascular conditions.
