Analytical Data
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Gene name
NOLA3
- Application
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Alternative Names
DKCB1; H/ACA ribonucleoprotein complex subunit 3; homolog of yeast Nop10p; MGC70651; NOLA3; NOP10; NOP10 ribonucleoprotein homolog (yeast); NOP10_HUMAN; NOP10P; Nucleolar protein 10; Nucleolar protein family A member 3; nucleolar protein family A. member 3 (H/ACA small nucleolar RNPs); snoRNP protein NOP10
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NPE3
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Expression Region
1-64 aa
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AA Sequence
MFLQYYLNEQ GDRVYTLKKF DPMGQQTCSA HPARFSPDDK YSRHRITIKK RFKVLMTQQP RPVL
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Molecular Weight
7.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NOLA3, also known as nucleolar protein 3, is a critical component of the nucleolus, playing an essential role in ribosome biogenesis and cellular stress responses. It is a member of the NOLA family of proteins, which are involved in the regulation of RNA processing and the assembly of ribonucleoprotein complexes. Research has indicated that NOLA3 may interact with various RNA molecules and other proteins, facilitating the maturation of ribosomal RNA (rRNA) and the formation of ribosomes, which are vital for protein synthesis in cells. The dysregulation of NOLA3 has been implicated in various cellular processes, including cell proliferation and apoptosis, suggesting its potential role in cancer and other diseases. Furthermore, studies have shown that NOLA3 expression levels can be modulated by different stressors, indicating its involvement in the cellular response to stress. Understanding the structure-function relationship of NOLA3 and its interactions is critical for elucidating its biological roles and could provide insights into therapeutic approaches for diseases associated with ribosome biogenesis and nucleolar dysfunction. Research into NOLA3 reconstitution seeks to clarify its mechanistic functions and interactions, paving the way for advancements in our understanding of nucleolar dynamics and their implications in health and disease.
