Analytical Data
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Gene name
NNT
- Application
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Alternative Names
GCCD4; mitochondrial; NAD(P) transhydrogenase; NAD(P) transhydrogenase mitochondrial; Nicotinamide nucleotide transhydrogenase; NNT; NNTM; NNTM_HUMAN; Pyridine nucleotide transhydrogenase
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13423
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Expression Region
1-207 aa
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AA Sequence
MNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK
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Molecular Weight
48.51 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NNT (Nicotinamide Nucleotide Transhydrogenase) is a crucial enzyme that plays a significant role in cellular metabolism, particularly in the mitochondrial functions of energy production and redox balance. Emerging research highlights its involvement in the regulation of NADH and NADPH levels, which are essential for various biochemical pathways, including those associated with oxidative stress and antioxidant defenses. The study of recombinant NNT protein is gaining traction due to its potential applications in understanding metabolic disorders, cardiovascular diseases, and aging-related conditions. By utilizing recombinant DNA technology, scientists aim to express and purify NNT protein, enabling detailed investigations of its structure, function, and interactions within mitochondrial pathways. This research could unveil the therapeutic potentials of modulating NNT activity, potentially leading to novel treatments for diseases linked to mitochondrial dysfunction. Furthermore, studying the regulatory mechanisms of NNT can provide insights into metabolic pathways and the development of strategies to enhance cellular resilience against oxidative damage. As such, NNT recombinant protein research represents a promising frontier in biomedical science, with implications for both basic research and clinical applications.
