Analytical Data
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Gene name
HSD17b2
- Application
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Alternative Names
HSD17b2；EDH17B2；SDR9C2；17-beta-hydroxysteroid dehydrogenase type 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P37059
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Expression Region
1-387aa
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AA Sequence
MSTFFSDTAWICLAVPTVLCGTVFCKYKKSSGQLWSWMVCLAGLCAVCLL ILSPFWGLILFSVSCFLMYTYLSGQELLPVDQKAVLVTGGDCGLGHALCK YLDELGFTVFAGVLNENGPGAEELRRTCSPRLSVLQMDITKPVQIKDAYS KVAAMLQDRGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFFGTVEVT KTFLPLLRKSKGRLVNVSSMGGGAPMERLASYGSSKAAVTMFSSVMRLEL SKWGIKVASIQPGGFLTNIAGTSDKWEKLEKDILDHLPAEVQEDYGQDYI LAQRNFLLLINSLASKDFSPVLRDIQHAILAKSPFAYYTPGKGAYLWICL AHYLPIGIYDYFAKRHFGQDKPMPRALRMPNYKKKAT
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Molecular Weight
42.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HSD17B2, or 17β-hydroxysteroid dehydrogenase type 2, is an important enzyme involved in the metabolism of steroid hormones, particularly in the interconversion of estrone (E1) and estradiol (E2). This enzyme plays a crucial role in regulating estrogen levels in different tissues, influencing various physiological processes such as reproduction, development, and bone health. Dysregulation of HSD17B2 has been linked to several conditions, including hormone-dependent cancers, cardiovascular diseases, and metabolic disorders, making it a significant target for therapeutic interventions. Recent studies have focused on the recombinant expression of HSD17B2 to better understand its enzymatic functions, elucidate its role in hormonal regulation, and develop potential inhibitors or modulators. The ability to produce HSD17B2 in a recombinant system allows for detailed biochemical characterization and structural studies, paving the way for the identification of key residues involved in substrate binding and catalysis. Additionally, understanding the structural and functional properties of this enzyme can facilitate drug design efforts aimed at treating diseases associated with estrogen imbalances. Overall, research on recombinant HSD17B2 is vital for advancing our knowledge of steroid hormone metabolism and its implications in human health and disease.
