Analytical Data
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基因名
EIF2B2
- Application
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别名
EIF2B2；EIF2BB；Translation initiation factor eIF2B subunit beta
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49770
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表达区间
1-351aa
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氨基酸序列
MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL
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分子量
66.0kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Eukaryotic translation initiation factor 2B (EIF2B) is a critical multi-subunit complex that plays a vital role in protein synthesis by facilitating the exchange of GDP for GTP on the initiation factor EIF2, thereby promoting the formation of the translation initiation complex. Among its five subunits, EIF2B2 is particularly noteworthy due to its implications in various cellular processes, including cell growth, differentiation, and stress responses. Mutations in EIF2B2 and other EIF2B subunits have been linked to neurological disorders, notably hypomyelinating leukodystrophy, a condition characterized by impaired myelin formation in the nervous system. This has propelled the scientific community's interest in understanding the functional mechanisms and structural properties of EIF2B2. Recent advances in recombinant protein technology allow researchers to produce and purify EIF2B2 in a laboratory setting, paving the way for detailed biochemical and biophysical studies. By elucidating the structure-function relationship of EIF2B2, scientists aim to uncover its regulatory mechanisms during translation initiation and its role in disease pathways. Such research not only contributes to our fundamental understanding of protein synthesis but may also inform the development of therapeutic strategies for disorders associated with EIF2B dysfunction.
