Analytical Data
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基因名
ALAS2
- Application
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别名
ALAS2；ALASE；ASB；5-aminolevulinate synthase. erythroid-specific. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P22557
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表达区间
50-587aa
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氨基酸序列
QIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFKTDLPSSLVSVSLRKPFSGPQEQEQISGKVTHLIQNNMPGNYVFSYDQFFRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSEASVASKDVSVWCSNDYLGMSRHPQVLQATQETLQRHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA
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分子量
63.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ALAS2 (aminolevulinic acid synthase 2) is an essential enzyme involved in heme biosynthesis, primarily expressed in erythroid cells. It catalyzes the condensation of glycine and succinyl-CoA to form aminolevulinic acid, the first step in the heme synthesis pathway. Mutations in the ALAS2 gene can lead to various forms of congenital erythropoietic porphyria and anemia, highlighting its significance in red blood cell development and function. Research into ALAS2 recombinant proteins serves multiple purposes, including the elucidation of the enzyme's structural and functional properties, exploring its regulatory mechanisms, and understanding its interactions with other proteins involved in heme metabolism. The production of ALAS2 as a recombinant protein allows for detailed biochemical assays, providing insights into its enzymatic activity and the effects of specific mutations on its function. Furthermore, studying ALAS2 can enhance our understanding of related pathologies and potentially lead to the development of therapeutic strategies for the management of diseases associated with heme synthesis disorders. Advances in recombinant protein technology have thus opened pathways for in-depth investigations into the role of ALAS2 in health and disease, making it a focal point in the field of molecular biology and clinical research.
