Analytical Data
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Gene name
ALAS2
- Application
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Alternative Names
ALAS2；ALASE；ASB；5-aminolevulinate synthase. erythroid-specific. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P22557
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Expression Region
50-587aa
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AA Sequence
QIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFKTDLPSSLVSVSLRKPFSGPQEQEQISGKVTHLIQNNMPGNYVFSYDQFFRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSEASVASKDVSVWCSNDYLGMSRHPQVLQATQETLQRHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA
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Molecular Weight
63.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ALAS2 (aminolevulinic acid synthase 2) is an essential enzyme involved in heme biosynthesis, primarily expressed in erythroid cells. It catalyzes the condensation of glycine and succinyl-CoA to form aminolevulinic acid, the first step in the heme synthesis pathway. Mutations in the ALAS2 gene can lead to various forms of congenital erythropoietic porphyria and anemia, highlighting its significance in red blood cell development and function. Research into ALAS2 recombinant proteins serves multiple purposes, including the elucidation of the enzyme's structural and functional properties, exploring its regulatory mechanisms, and understanding its interactions with other proteins involved in heme metabolism. The production of ALAS2 as a recombinant protein allows for detailed biochemical assays, providing insights into its enzymatic activity and the effects of specific mutations on its function. Furthermore, studying ALAS2 can enhance our understanding of related pathologies and potentially lead to the development of therapeutic strategies for the management of diseases associated with heme synthesis disorders. Advances in recombinant protein technology have thus opened pathways for in-depth investigations into the role of ALAS2 in health and disease, making it a focal point in the field of molecular biology and clinical research.
