Analytical Data
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Gene name
NDUFA9
- Application
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Alternative Names
CI-39kD; Complex I subunit NDUFA9; Complex I-39kD; mitochondrial; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 mitochondrial; NADH Ubiquinone Oxidoreductase 1 alpha subcomplex 9; NADH-ubiquinone oxidoreductase 39 kDa subunit; NDUA9_HUMAN; NDUFA9
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16795
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Expression Region
36-377 aa
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AA Sequence
LHHAL MPHGKGGRSS VSGIVATVFG ATGFLGRYVV NHLGRMGSQV IIPYRCDKYD IMHLRPMGDL GQLLFLEWDA RDKDSIRRVV QHSNVVINLI GRDWETKNFD FEDVFVKIPQ AIAQLSKEAG VEKFIHVSHL NANIKSSSRY LRNKAVGEKV VRDAFPEAII VKPSDIFGRE DRFLNSFASM HRFGPIPLGS LGWKTVKQPV YVVDVSKGIV NAVKDPDANG KSFAFVGPSR YLLFHLVKYI FAVAHRLFLP FPLPLFAYRW VARVFEISPF EPWITRDKVE RMHITDMKLP HLPGLEDLGI QATPLELKAI EVLRRHRTYR WLSAEIEDVK PAKTVNI
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Molecular Weight
42.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDUFA9 is a subunit of the mitochondrial NADH:ubiquinone oxidoreductase, also known as Complex I, which plays a critical role in the mitochondrial respiratory chain, facilitating the transfer of electrons from NADH to ubiquinone and contributing to ATP synthesis. Research into NDUFA9 is gaining momentum due to its involvement in various mitochondrial diseases and its potential link to neurodegenerative disorders, such as Parkinson's disease. Mutations or deficiencies in this subunit can lead to impaired mitochondrial function, resulting in reduced ATP production and increased oxidative stress, which is detrimental to cellular health. Recombinant NDUFA9 protein has become a valuable tool for studying the structure-function relationships in Complex I, enabling insights into the molecular mechanisms underlying its role in energy metabolism. Furthermore, understanding the dynamics of NDUFA9 may provide therapeutic avenues for targeting mitochondrial dysfunction and developing strategies for disease intervention. Current research is also exploring the applications of NDUFA9 in the context of bioenergetics, cellular signaling, and as a potential biomarker for mitochondrial diseases. As such, the study of recombinant NDUFA9 holds significant promise for elucidating the complexities of mitochondrial biology and its implications for human health and disease.
