Analytical Data
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Gene name
NDUFA8
- Application
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Alternative Names
CI 19KD; CI PGIV; CI-19kD; CI-PGIV; Complex I 19k; Complex I 19kD; Complex I PGIV; Complex I PGIV subunit; Complex I-19kD; Complex I-PGIV; MGC793; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 (19kD PGIV); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 19kDa; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; NADH ubiquinone oxidoreductase 19 kDa subunit; NADH ubiquinone oxidoreductase PGIV subunit ; NADH-ubiquinone oxidoreductase 19 kDa subunit; NDUA8_HUMAN; NDUFA 8; NDUFA8; OTTHUMP00000022034; PGIV
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51970
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Expression Region
2-172 aa
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AA Sequence
PGIVELPTL EELKVDEVKI SSAVLKAAAH HYGAQCDKPN KEFMLCRWEE KDPRRCLEEG KLVNKCALDF FRQIKRHCAE PFTEYWTCID YTGQQLFRHC RKQQAKFDEC VLDKLGWVRP DLGELSKVTK VKTDRPLPEN PYHSRPRPDP SPEIEGDLQP ATHGSRFYFW TK
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Molecular Weight
20.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDUFA8, a key subunit of the mitochondrial NADH dehydrogenase complex I, plays a crucial role in the electron transport chain, which is vital for ATP production through oxidative phosphorylation. Dysfunction of complex I is linked to various mitochondrial disorders and has been implicated in several neurodegenerative diseases, such as Parkinson's and Alzheimer's. Given the importance of NDUFA8 in maintaining mitochondrial integrity and energy metabolism, researchers are increasingly focusing on its biochemical properties, structural attributes, and interactions with other mitochondrial components. The study of recombinant NDUFA8 protein offers insights into its functional mechanisms, potential regulatory roles, and the impact of mutations on complex I activity. Advances in recombinant protein technology enable the production of NDUFA8 in a controlled environment, facilitating detailed analyses through biochemical assays and structural studies. Understanding the intricate functions of NDUFA8 can aid in developing therapeutic strategies aimed at mitochondrial dysfunction and related diseases, highlighting its significance in both basic and translational research within the field of mitochondrial biology.
