Analytical Data
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Gene name
JA
- Application
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Alternative Names
JA;Eif3s1-1;Eukaryotic translation initiation factor 3 subunit J-A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75822
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Expression Region
2-258aa
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AA Sequence
AAAAAAAGD SDSWDADAFS VEDPVRKVGG GGTAGGDRWE GEDEDEDVKD NWDDDDDEKK EEAEVKPEVK ISEKKKIAEK IKEKERQQKK RQEEIKKRLE EPEEPKVLTP EEQLADKLRL KKLQEESDLE LAKETFGVNN AVYGIDAMNP SSRDDFTEFG KLLKDKITQY EKSLYYASFL EVLVRDVCIS LEIDDLKKIT NSLTVLCSEK QKQEKQSKAK KKKKGVVPGG GLKATMKDDL ADYGGYDGGY VQDYEDFM
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Molecular Weight
29 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
JA (Jasmonic Acid) is a key plant hormone involved in regulating various physiological processes, including growth, germination, and defense mechanisms against biotic and abiotic stresses. The JA signaling pathway is activated in response to wounding, insect attack, and pathogen infection, leading to a range of protective responses. Research into JA has grown significantly due to its vital role in plant defense and potential applications in agriculture, such as enhancing crop resilience and improving yield. The study of JA-recombinant proteins involves the expression and purification of protein products that are either directly involved in the JA signaling pathway or serve as tools for further functional analysis. This research aims to elucidate the molecular mechanisms underlying JA perception and signal transduction, thus providing insights into plant hormone interactions and their effects on plant development. The advances in molecular biology techniques, including genetic engineering and CRISPR technology, have facilitated the production of JA-recombinant proteins. These proteins are critical for studying the specific roles of JA-related genes and their regulatory networks, ultimately contributing to the development of bioengineering strategies to improve stress tolerance in crops. As such, understanding JA and its associated proteins holds great promise for enhancing sustainable agricultural practices in the face of global challenges like climate change and food security.











