Analytical Data
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Gene name
CASQ
- Application
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Alternative Names
CASQ；CASQ；Calsequestrin-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P31415
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Expression Region
35-396aa
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AA Sequence
QEGLDF PEYDGVDRVI NVNAKNYKNV FKKYEVLALL YHEPPEDDKA SQRQFEMEEL ILELAAQVLE DKGVGFGLVD SEKDAAVAKK LGLTEVDSMY VFKGDEVIEY DGEFSADTIV EFLLDVLEDP VELIEGEREL QAFENIEDEI KLIGYFKSKD SEHYKAFEDA AEEFHPYIPF FATFDSKVAK KLTLKLNEID FYEAFMEEPV TIPDKPNSEE EIVNFVEEHR RSTLRKLKPE SMYETWEDDM DGIHIVAFAE EADPDGFEFL ETLKAVAQDN TENPDLSIIW IDPDDFPLLV PYWEKTFDID LSAPQIGVVN VTDADSVWME MDDEEDLPSA EELEDWLEDV LEGEINTEDD DDDDDD
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Molecular Weight
45.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CASQ (Calsequestrin) proteins are intracellular calcium-binding proteins that play a crucial role in the regulation of calcium homeostasis within muscle cells, particularly in the sarcoplasmic reticulum of skeletal and cardiac muscles. The study of CASQ proteins has garnered significant attention due to their essential function in muscle contraction and relaxation processes, making them vital for normal muscle function. Mutations and dysregulation of CASQ proteins have been implicated in a variety of muscle-related diseases, including congenital myopathies and cardiac dysfunctions. Researchers have focused on characterizing the structure and functional properties of CASQ proteins to understand their mechanisms at a molecular level. Recombinant expression of CASQ proteins allows for detailed studies of their biophysical properties and interactions with other proteins involved in calcium signaling pathways. This research is critical for developing therapeutic strategies for muscle diseases, as understanding the intricate role of CASQ proteins may lead to targeted treatments that can restore calcium homeostasis and improve muscle function. Overall, the investigation of CASQ recombinant proteins represents a promising avenue for unraveling the complexities of calcium signaling in muscle physiology and pathophysiology.
