Analytical Data
-
Gene name
RFC4
- Application
-
Alternative Names
RFC4；Replication factor C subunit 4
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P35249
-
Expression Region
1-363aa
-
AA Sequence
MQAFLKGTSI STKPPLTKDR GVAASAGSSG ENKKAKPVPW VEKYRPKCVD EVAFQEEVVA VLKKSLEGAD LPNLLFYGPP GTGKTSTILA AARELFGPEL FRLRVLELNA SDERGIQVVR EKVKNFAQLT VSGSRSDGKP CPPFKIVILD EADSMTSAAQ AALRRTMEKE SKTTRFCLIC NYVSRIIEPL TSRCSKFRFK PLSDKIQQQR LLDIAKKENV KISDEGIAYL VKVSEGDLRK AITFLQSATR LTGGKEITEK VITDIAGVIP AEKIDGVFAA CQSGSFDKLE AVVKDLIDEG HAATQLVNQL HDVVVENNLS DKQKSIITEK LAEVDKCLAD GADEHLQLIS LCATVMQQLS QNC
-
Molecular Weight
39.6 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RFC4, or Replication Factor C subunit 4, is a crucial component of the Replication Factor C complex, which plays a significant role in DNA replication and repair processes. As a part of this complex, RFC4 facilitates the loading of the sliding clamp, PCNA (Proliferating Cell Nuclear Antigen), onto DNA during replication, thereby enhancing the processivity of DNA polymerases. Research into RFC4 has gained momentum due to its implications in cellular proliferation, tumorigenesis, and various DNA damage responses. Abnormal expression or malfunction of RFC4 has been linked to several types of cancer, underscoring its potential as a biomarker for tumor progression and a target for therapeutic interventions. Furthermore, understanding the structural and functional dynamics of RFC4 can provide insights into the regulation of DNA replication and associated pathways, making it a vital element in cancer biology and molecular genetics. Current studies are focusing on unraveling its mechanisms of action, interactions with other protein complexes, and its role in maintaining genomic stability, highlighting its significance in both fundamental research and clinical applications.
