Analytical Data
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Gene name
POLR2I
- Application
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Alternative Names
POLR2I;DNA-directed RNA polymerase II subunit RPB9
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P36954
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Expression Region
1-125aa
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AA Sequence
MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN SCIYVNKITH EVDELTQIIA DVSQDPTLPR TEDHPCQKCG HKEAVFFQSH SARAEDAMRL YYVCTAPHCG HRWTE
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of POLR2I, a core subunit of RNA polymerase II (RNAP II), is crucial for understanding the intricate mechanisms of transcription regulation in eukaryotic cells. RNA polymerase II is responsible for synthesizing messenger RNA (mRNA) and plays a vital role in gene expression and regulation. POLR2I, also known as RPB5, is integral to the assembly and stability of the polymerase complex, influencing the transcriptional activity of a myriad of genes. Research into POLR2I has gained momentum due to its involvement in various cellular processes and pathological conditions, including cancer and neurodegenerative diseases. Understanding the structure and function of POLR2I through recombinant protein studies provides insights into its interactions with other transcription factors and cofactors, elucidating its role in the transcription machinery. Furthermore, studying POLR2I can shed light on the potential development of therapeutic targets, as dysregulation of transcriptional processes often leads to disease states. Advances in recombinant protein techniques have facilitated the analysis of POLR2I’s properties, making it an attractive candidate for further exploration in transcription-related research. Overall, investigating POLR2I not only enhances our understanding of fundamental biological processes but also holds promise for innovative approaches in treating transcription-related diseases.
