Analytical Data
-
基因名
METTL3
- Application
-
别名
METTL3；MTA70；N6-adenosine-methyltransferase catalytic subunit
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q86U44
-
表达区间
2-580aa
-
氨基酸序列
SDTWSSIQAHKKQLDSLRERLQRRRKQDSGHLDLRNPEAALSPTFRSDSPVPTAPTSGGPKPSTASAVPELATDPELEKKLLHHLSDLALTLPTDAVSICLAISTPDAPATQDGVESLLQKFAAQELIEVKRGLLQDDAHPTLVTYADHSKLSAMMGAVAEKKGPGEVAGTVTGQKRRAEQDSTTVAAFASSLVSGLNSSASEPAKEPAKKSRKHAASDVDLEIESLLNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEKFRSRGRAQVQEFCDYGTKEECMKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVRSTSHKPDEIYGMIERLSPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFKQRYPDGIISKPKNL
-
分子量
70.4 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
METTL3, an integral component of the m6A methyltransferase complex, has garnered significant attention in recent years due to its pivotal role in the regulation of RNA methylation, which impacts various biological processes including mRNA stability, splicing, translation, and degradation. This enzyme catalyzes the methylation of the N6 position of adenosine residues in RNA, thereby influencing gene expression and cellular responses to environmental cues. The dysregulation of METTL3 has been associated with several diseases, including cancer, neurological disorders, and metabolic syndromes, highlighting its potential as a therapeutic target. Research on METTL3 recombinant proteins primarily focuses on understanding its enzymatic mechanisms, the identification of its substrates, and the exploration of its interactions with other cellular factors. Such studies are crucial for elucidating the broader implications of m6A methylation in cellular homeostasis and pathogenesis. Furthermore, the development of METTL3 inhibitors or modulators could provide novel strategies for disease intervention, thereby making it a central topic in molecular biology and biomedicine. As a result, the investigation into METTL3 recombinant proteins not only advances our comprehension of RNA metabolism but also paves the way for innovative therapeutic approaches to target diseases associated with aberrant RNA methylation.
