Analytical Data
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Gene name
BCAT2
- Application
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Alternative Names
BCAT2；BCATM；BCT2；ECA40；Branched-chain-amino-acid aminotransferase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15382
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Expression Region
28-392aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSHMASSSFKAADLQLEMTQKPHKKPGPG EPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLF EGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLI EVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFP GGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQV LWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLL DMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILY KDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV
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Molecular Weight
44 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BCAT2 (branched-chain amino acid transaminase 2) is an important enzyme involved in the metabolism of branched-chain amino acids (BCAAs) like leucine, isoleucine, and valine, which are crucial for various physiological functions, including protein synthesis and energy production. Dysregulation of BCAT2 has been implicated in several metabolic disorders and diseases, including obesity, diabetes, and certain types of cancer. Understanding the structure and function of BCAT2 is essential for elucidating its role in these diseases and developing potential therapeutic interventions. Research on the recombinant expression of BCAT2 has gained momentum due to its potential applications in biotechnology and pharmaceuticals. By producing and purifying BCAT2 as a recombinant protein, scientists aim to explore its enzymatic properties, regulatory mechanisms, and interaction with other metabolic pathways. Recombinant BCAT2 can serve as a valuable tool for studying its biological function in vitro and in vivo, as well as for screening small molecules that may modulate its activity. The insights gained from these studies could lead to novel strategies for treating metabolic disorders associated with altered BCAA metabolism, highlighting the significance of BCAT2 research in the broader context of metabolic health.
