Analytical Data
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Gene name
METTL5
- Application
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Alternative Names
2810410A08Rik; DC3; FLJ10459; HSPC133; mCG_12967; Methyltransferase like protein 5; Methyltransferase-like protein 5; METL5_HUMAN; METTL 5; METTL5; MGC187943; OTTHUMP00000205107; OTTHUMP00000205108; OTTHUMP00000205109; OTTMUSP00000040951; RGD1566062
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NRN9
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Expression Region
1-209 aa
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AA Sequence
MKKVRLKELE SRLQQVDGFE KPKLLLEQYP TRPHIAACML YTIHNTYDDI ENKVVADLGC GCGVLSIGTA MLGAGLCVGF DIDEDALEIF NRNAEEFELT NIDMVQCDVC LLSNRMSKSF DTVIMNPPFG TKNNKGTDMA FLKTALEMAR TAVYSLHKSS TREHVQKKAA EWKIKIDIIA ELRYDLPASY KFHKKKSVDI EVDLIRFSF
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Molecular Weight
23.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
METTL5 is a methyltransferase enzyme that plays a critical role in the post-transcriptional modification of RNA. It is known to specifically catalyze the N6-methyladenosine (m6A) modification, a crucial epitranscriptomic mark that influences various aspects of RNA metabolism, including splicing, stability, translation, and degradation. Recent studies suggest that METTL5 is involved in regulating gene expression and cellular processes, with implications in development, differentiation, and disease progression. The dysfunction of m6A modifications, particularly through dysregulation of METTL5, has been linked to numerous disorders, including cancer and neurological diseases. As research progresses, understanding the molecular mechanisms through which METTL5 operates could provide insights into therapeutic targets and strategies for manipulating RNA modifications in disease contexts. Given its significance, the recombinant production of METTL5 is crucial for conducting structural and functional studies, enabling researchers to explore its enzymatic activity, substrate specificity, and interactions with other cellular factors. This knowledge can further elucidate the role of METTL5 in the intricate regulatory networks governing RNA metabolism and offer potential avenues for biotechnological and clinical applications.
