Analytical Data
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Gene name
PDK1
- Application
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Alternative Names
PDK1;PDHK1;[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15118
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Expression Region
2-436aa
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AA Sequence
RLARLLRGA ALAGPGPGLR AAGFSRSFSS DSGSSPASER GVPGQVDFYA RFSPSPLSMK QFLDFGSVNA CEKTSFMFLR QELPVRLANI MKEISLLPDN LLRTPSVQLV QSWYIQSLQE LLDFKDKSAE DAKAIYDFTD TVIRIRNRHN DVIPTMAQGV IEYKESFGVD PVTSQNVQYF LDRFYMSRIS IRMLLNQHSL LFGGKGKGSP SHRKHIGSIN PNCNVLEVIK DGYENARRLC DLYYINSPEL ELEELNAKSP GQPIQVVYVP SHLYHMVFEL FKNAMRATME HHANRGVYPP IQVHVTLGNE DLTVKMSDRG GGVPLRKIDR LFNYMYSTAP RPRVETSRAV PLAGFGYGLP ISRLYAQYFQ GDLKLYSLEG YGTDAVIYIK ALSTDSIERL PVYNKAAWKH YNTNHEADDW CVPSREPKDM TTFRSA
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Molecular Weight
72 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDK1 (3-phosphoinositide-dependent protein kinase-1) is a pivotal serine/threonine kinase that plays a crucial role in various cellular processes, including metabolism, cell growth, survival, and differentiation. It is a key component of the phosphoinositide signaling pathway and acts as an upstream regulator of the AKT signaling pathway, which is frequently implicated in cancer and other diseases. Research into PDK1 has garnered significant interest due to its involvement in various pathophysiological conditions, including cancer progression and insulin signaling pathways. The recombination of PDK1 offers a valuable tool for studying its function and regulation at a molecular level. By producing recombinant PDK1 protein, researchers can investigate its kinase activity, interaction with other signaling molecules, and its structural characteristics, which may provide insights into the development of targeted therapies for diseases associated with dysregulated PDK1 activity. Furthermore, the study of PDK1 and its interactions can reveal potential mechanisms of signal transduction that are critical for cellular responses to external stimuli. Understanding these intricate pathways can aid in the discovery of novel therapeutic strategies to combat metabolic disorders and cancers driven by abnormal signaling processes. Therefore, the investigation of recombinant PDK1 is essential for elucidating its function in health and disease, positioning it as a promising target for future therapeutic interventions.
