Analytical Data
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Gene name
MCM10
- Application
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Alternative Names
CNA43; DNA43; FLJ31133; Homolog of yeast MCM10; HsMCM10; mcm10; MCM10 minichromosome maintenance deficient 10; MCM10_HUMAN; Minichromosome maintenance 10 replication initiation factor; Minichromosome maintenance complex component 10; Minichromosome maintenance protein 10 isoform 1; PRO2249; Protein MCM10 homolog
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7L590
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Expression Region
1-874aa
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AA Sequence
MDEEEDNLSLLTALLEENESALDCNSEENNFLTRENGEPDAFDELFDADGDGESYTEEADDGETGETRDEKENLATLFGDMEDLTDEEEVPASQSTENRVLPAPAPRREKTNEELQEELRNLQEQMKALQEQLKVTTIKQTASPARLQKSPEKSPRPPLKERRVQRIQESTCFSAELDVPALPRTKRVARTPKASPPDPKSSSSRMTSAPSQPLQTISRNKPSGITRGQIVGTPGSSGETTQPICVEAFSGLRLRRPRVSSTEMNKKMTGRKLIRLSQIKEKMAREKLEEIDWVTFGVILKKVTPQSVNSGKTFSIWKLNDLRDLTQCVSLFLFGEVHKALWKTEQGTVVGILNANPMKPKDGSEEVCLSIDHPQKVLIMGEALDLGTCKAKKKNGEPCTQTVNLRDCEYCQYHVQAQYKKLSAKRADLQSTFSGGRIPKKFARRGTSLKERLCQDGFYYGGVSSASYAASIAAAVAPKKKIQTTLSNLVVKGTNLIIQETRQKLGIPQKSLSCSEEFKELMDLPTCGARNLKQHLAKATASGIMGSPKPAIKSISASALLKQQKQRMLEMRRRKSEEIQKRFLQSSSEVESPAVPSSSRQPPAQPPRTGSEFPRLEGAPATMTPKLGRGVLEGDDVLFYDESPPPRPKLSALAEAKKLAAITKLRAKGQVLTKTNPNSIKKKQKDPQDILEVKERVEKNTMFSSQAEDELEPARKKRREQLAYLESEEFQKILKAKSKHTGILKEAEAEMQERYFEPLVKKEQMEEKMRNIREVKCRVVTCKTCAYTHFKLLETCVSEQHEYHWHDGVKRFFKCPCGNRSISLDRLPNKHCSNCGLYKWERDGMLKEKTGPKIGGETLLPRGEEHAKFLNSLK
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Molecular Weight
124.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MCM10, a member of the minichromosome maintenance (MCM) family, plays a crucial role in the process of DNA replication and is essential for the maintenance of genome stability. As a helicase loader, MCM10 assists in the recruitment and stabilization of the MCM2-7 helicase complex at replication origins, facilitating its activation during the initiation of DNA synthesis. Dysregulation of MCM10 has been implicated in various cancers, highlighting its potential as a biomarker for tumor progression and a target for therapeutic interventions. The study of MCM10 and its interactions within the replicative machinery is vital for understanding the molecular mechanisms of DNA replication, the factors that contribute to genomic instability, and the development of cancer. Recent research efforts have focused on elucidating the structure-function relationships of MCM10, its regulatory mechanisms during the cell cycle, and its potential roles beyond replication, such as in DNA repair and damage responses. Emerging evidence suggests that MCM10's function may also be linked to other cellular processes, making it a significant focus of investigation in cancer biology and molecular genetics.
