Analytical Data
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基因名
BLM
- Application
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别名
BLM；RECQ2；RECQL3；RecQ-like DNA helicase BLM
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P54132
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表达区间
877-1024aa
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氨基酸序列
DCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLY
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分子量
23.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of BLM (Bloom syndrome helicase) recombinant proteins has garnered significant interest due to the pivotal role that BLM plays in maintaining genomic stability and preventing cancer. BLM is a member of the RecQ helicase family and is crucial for DNA replication, repair, and homologous recombination. Mutations in the BLM gene lead to Bloom syndrome, a rare genetic disorder characterized by growth deficiencies, immunodeficiency, and a markedly increased risk of various cancers. Understanding the biochemical properties and functional mechanisms of BLM through recombinant protein studies allows researchers to elucidate its specific roles in DNA metabolism and repair pathways. By producing recombinant BLM proteins, scientists can investigate their enzymatic activities, interactions with other proteins, and the effects of specific mutations. These studies are essential not only for comprehending the basic cellular processes governed by BLM but also for developing potential therapeutic strategies to tackle disorders linked to genomic instability and cancer. As such, the investigation of BLM recombinant proteins continues to be a rich area of research with important implications for both basic science and clinical applications in oncology and genetics.
