Analytical Data
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Gene name
PBK
- Application
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Alternative Names
PBK;TOPK;Lymphokine-activated killer T-cell-originated Protein kinase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96KB5
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Expression Region
1-322aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMEGISN FKTPSKLSEK KKSVLCSTPT INIPASPFMQ KLGFGTGVNV YLMKRSPRGL SHSPWAVKKI NPICNDHYRS VYQKRLMDEA KILKSLHHPN IVGYRAFTEA NDGSLCLAME YGGEKSLNDL IEERYKASQD PFPAAIILKV ALNMARGLKY LHQEKKLLHG DIKSSNVVIK GDFETIKICD VGVSLPLDEN MTVTDPEACY IGTEPWKPKE AVEENGVITD KADIFAFGLT LWEMMTLSIP HINLSNDDDD EDKTFDESDF DDEAYYAALG TRPPINMEEL DESYQKVIEL FSVCTNEDPK DRPSAAHIVE ALETDV
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Molecular Weight
39 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of PBK (PDZ-binding kinase) recombinant proteins has gained considerable attention due to the kinase's role in various cellular processes, including cell proliferation, survival, and migration. PBK, also known as T-LAK cell-originated protein kinase (TOPK), is a serine/threonine kinase that belongs to the family of mitogen-activated protein (MAP) kinases. Research has indicated that PBK is involved in the progression of several cancers, making it a potential target for therapeutic intervention. The recombinant production of PBK allows for detailed biochemical characterization and functional studies, enabling researchers to explore its mechanisms of action and regulatory pathways in cell signaling. Advances in protein expression and purification techniques have made it feasible to obtain large quantities of functional PBK, facilitating experiments that elucidate its structure-function relationship and interactions with other proteins. Moreover, understanding PBK's involvement in oncogenesis may lead to the development of novel cancer treatments, highlighting the importance of this area of research in both basic biology and clinical applications. As such, the investigation of PBK recombinant proteins stands at the forefront of molecular biology studies focused on cancer therapeutics and cell signaling pathways.
