Analytical Data
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Gene name
sph2
- Application
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Alternative Names
sph2；SK2；Sphingosine kinase 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P59116
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Expression Region
1-623aa
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AA Sequence
MINKITKPKLLIGYYLLLFSLIRCLPEKESSYKDLFTSLLFLPNQTNSNQVNSVSINNDPANPNPVNPASANNNQVNAVPENDDPANLNPVNPASANSNQVNAAPENGSPADPNPANLASANNNQVNAVPANNYFTKEDSSNNIPKKVNSKNVEIKVLSHNVFMLPTNLPRWGNLGHDERAKRISKSDYVKNQDVIVFEEAFDTSARKILLDNLREEYPYQTDVVGRTKKNWDASLGNFRSYSLVNGGVVILSKWPIEEKIQYIFNDSGCGADWFANKGFVYVKINKEGKKFHVIGTHAQSQDQNCSNLGIPNRANQFDDIRNFIYSKNIPKDETVLIVGDLNVIKESNEYYDMISRLNVNEPRYVGVPFTWDAKTNEIAAYYYENEEPVYLDYIFVSKSHAQPPVWQNLAYDPVSKQTWTVSGYTSDEFSDHYPIYGFVYADPSTPTKSGHKKKYDQVSFQSAANGKYIQADPNRKNGWLKADAVIETDFTKFNLLQEGNLNPSCIKNGLVRIESSRFLNYFWNWWLGGGSGNYGYYSKFNDASNQLEIINLSDECLENGSKIVFKDYDTYSRNHYYLTVWDKGNWNEHLYLWKDSISQREIFYLKLNSTPVRNWSADLIYR
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Molecular Weight
71kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on sph2 recombinant protein primarily stems from the increasing interest in the functional roles of sphingolipids in cellular processes and their implications in various diseases. Sph2, a sphingolipid-derived molecule, has been linked to critical biological functions such as cell signaling, apoptosis, and inflammation. The ability to express sph2 as a recombinant protein facilitates detailed biochemical studies, enabling scientists to analyze its structure-function relationship, interactions with other biomolecules, and its potential as a therapeutic target. Recent advancements in recombinant DNA technology have allowed for more efficient production and purification of sph2, making it accessible for in vitro and in vivo studies. As sphingolipid metabolism continues to be a promising field in understanding neurodegenerative diseases, cancer, and metabolic disorders, the exploration of sph2 recombinant protein may uncover novel insights into disease mechanisms, thus opening new avenues for targeted therapies and diagnostic tools. The ongoing efforts to investigate sph2's role within these contexts underscore its potential significance in both basic and applied biomedical research.
