Analytical Data
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基因名
sph2
- Application
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别名
sph2；SK2；Sphingosine kinase 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P59116
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表达区间
1-623aa
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氨基酸序列
MINKITKPKLLIGYYLLLFSLIRCLPEKESSYKDLFTSLLFLPNQTNSNQVNSVSINNDPANPNPVNPASANNNQVNAVPENDDPANLNPVNPASANSNQVNAAPENGSPADPNPANLASANNNQVNAVPANNYFTKEDSSNNIPKKVNSKNVEIKVLSHNVFMLPTNLPRWGNLGHDERAKRISKSDYVKNQDVIVFEEAFDTSARKILLDNLREEYPYQTDVVGRTKKNWDASLGNFRSYSLVNGGVVILSKWPIEEKIQYIFNDSGCGADWFANKGFVYVKINKEGKKFHVIGTHAQSQDQNCSNLGIPNRANQFDDIRNFIYSKNIPKDETVLIVGDLNVIKESNEYYDMISRLNVNEPRYVGVPFTWDAKTNEIAAYYYENEEPVYLDYIFVSKSHAQPPVWQNLAYDPVSKQTWTVSGYTSDEFSDHYPIYGFVYADPSTPTKSGHKKKYDQVSFQSAANGKYIQADPNRKNGWLKADAVIETDFTKFNLLQEGNLNPSCIKNGLVRIESSRFLNYFWNWWLGGGSGNYGYYSKFNDASNQLEIINLSDECLENGSKIVFKDYDTYSRNHYYLTVWDKGNWNEHLYLWKDSISQREIFYLKLNSTPVRNWSADLIYR
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分子量
71kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The research on sph2 recombinant protein primarily stems from the increasing interest in the functional roles of sphingolipids in cellular processes and their implications in various diseases. Sph2, a sphingolipid-derived molecule, has been linked to critical biological functions such as cell signaling, apoptosis, and inflammation. The ability to express sph2 as a recombinant protein facilitates detailed biochemical studies, enabling scientists to analyze its structure-function relationship, interactions with other biomolecules, and its potential as a therapeutic target. Recent advancements in recombinant DNA technology have allowed for more efficient production and purification of sph2, making it accessible for in vitro and in vivo studies. As sphingolipid metabolism continues to be a promising field in understanding neurodegenerative diseases, cancer, and metabolic disorders, the exploration of sph2 recombinant protein may uncover novel insights into disease mechanisms, thus opening new avenues for targeted therapies and diagnostic tools. The ongoing efforts to investigate sph2's role within these contexts underscore its potential significance in both basic and applied biomedical research.
