Analytical Data
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Gene name
EN2
- Application
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Alternative Names
EN2；Homeobox Protein engrailed-2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P19622
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Expression Region
86-210aa
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AA Sequence
GTCCAGAGGGRGGGAGGEGGASGAEGGGGAGGSEQLLGSGSREPRQNPPC APGAGGPLPAAGSDSPGDGEGGSKTLSLHGGAKKGGDPGGPLDGSLKARG LGGGDLSVSSDSDSSQAGANLGAQP
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Molecular Weight
34.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The EN2 (Engrailed-2) protein is a homeodomain transcription factor that plays a crucial role in the development and patterning of the central nervous system and limb structures in vertebrates. Researchers have focused on EN2 due to its implications in various neurodevelopmental disorders and its potential as a biomarker for specific cancers, particularly in the context of the brain and prostate. The protein is also essential in regulating gene expression during embryogenesis, with its malfunction linked to pathologies such as autism spectrum disorders and schizophrenia. Recent studies have sought to explore the structural properties of EN2, its interaction with other proteins, and its role in cellular signaling pathways. Recombination techniques have enabled scientists to produce EN2 in a laboratory setting, allowing for deeper analysis of its functional domains and regulatory mechanisms. By utilizing recombinant DNA technology, researchers can generate EN2 variants to study how specific mutations affect its function and pathways. The growing interest in EN2 research underscores its significance not only in developmental biology but also in the field of therapeutic interventions, as a better understanding of this protein could lead to novel approaches in treating conditions associated with its dysregulation. Through various experimental models, scientists aim to elucidate EN2's complex interactions within the cellular milieu, paving the way for advancements in both basic research and clinical applications.
