Analytical Data
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Gene name
SPT14
- Application
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Alternative Names
SPT14；CWH6；GPI3；Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P32363
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Expression Region
1-377aa
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AA Sequence
MGFNIAMLCDFFYPQLGGVEFHIYHLSQKLIDLGHSVVIITHAYKDRVGVRHLTNGLKVYHVPFFVIFRETTFPTVFSTFPIIRNILLREQIQIVHSHGSASTFAHEGILHANTMGLRTVFTDHSLYGFNNLTSIWVNKLLTFTLTNIDRVICVSNTCKENMIVRTELSPDIISVIPNAVVSEDFKPRDPTGGTKRKQSRDKIVIVVIGRLFPNKGSDLLTRIIPKVCSSHEDVEFIVAGDGPKFIDFQQMIESHRLQKRVQLLGSVPHEKVRDVLCQGDIYLHASLTEAFGTILVEAASCNLLIVTTQVGGIPEVLPNEMTVYAEQTSVSDLVQATNKAINIIRSKALDTSSFHDSVSKMYDWMDVAKRTVEIYTN
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Molecular Weight
49.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SPT14, also known as Suppressor of Ty 14, is a protein that plays a critical role in various cellular processes, including transcriptional regulation and stress response. Research on SPT14 has gained significant attention due to its involvement in chromatin remodeling and gene expression. Specifically, studies have shown that SPT14 is a key component of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, which is essential for histone acetylation and transcriptional activation. The understanding of SPT14's function has implications in developmental biology, cancer research, and the study of hereditary diseases, where dysregulation of gene expression can lead to pathological conditions. Recent advances in recombinant protein technology have enabled the production and purification of SPT14, facilitating in-depth biochemical characterization and functional assays. By examining how SPT14 interacts with other components of the transcription machinery and understanding its structural features, researchers aim to elucidate its precise role in cellular homeostasis. The insights gained from SPT14 studies could pave the way for novel therapeutic strategies targeting gene regulation and transcriptional misfires in various diseases, making it a focal point in contemporary molecular biology research.
