Analytical Data
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Gene name
NT5M
- Application
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Alternative Names
NT5M;DNT2;5'(3')-deoxyribonucleotidase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NPB1
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Expression Region
32-228aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMGGRALRVLVDMDGVLADFEGGFLRKFRAR FPDQPFIALEDRRGFWVS EQYGRLRPGLSEKAISIWESKNFFFELEPL PGAVEAVKEMASLQNTDVFICTSPIKMFKYCPYEKYAWVEKYFGP DFL EQIVLTRDKTVVSADLLIDDRPDITGAEPTPSWEHVLFTACHNQHLQLQP PRRRLHSWADDWKAILDSKRPC
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Molecular Weight
25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NT5M, or cytosolic 5'-nucleotidase, is an enzyme implicated in nucleotide metabolism, playing a crucial role in the dephosphorylation of nucleotides, particularly in the regulation of cellular nucleotide pools. Given its significant function in purine and pyrimidine metabolism, NT5M has emerged as a focal point in various biological and clinical research areas, including cancer biology, cellular signaling, and metabolic disorders. Abnormal expression or activity of NT5M has been associated with several pathological conditions, suggesting its potential as a biomarker or therapeutic target. Recent studies have utilized recombinant NT5M proteins to investigate its enzymatic properties, substrate specificity, and role in cellular signaling pathways. Moreover, understanding the structural dynamics of NT5M through reconstitution and characterization of recombinant forms has provided insights into its regulatory mechanisms and interactions with other cellular components. Such research is pivotal for elucidating the biochemical mechanisms underlying cellular metabolism and for the development of targeted therapies in diseases where nucleotide metabolism is dysregulated.
