Analytical Data
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基因名
NT5M
- Application
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别名
NT5M;DNT2;5'(3')-deoxyribonucleotidase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NPB1
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表达区间
32-228aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGGRALRVLVDMDGVLADFEGGFLRKFRAR FPDQPFIALEDRRGFWVS EQYGRLRPGLSEKAISIWESKNFFFELEPL PGAVEAVKEMASLQNTDVFICTSPIKMFKYCPYEKYAWVEKYFGP DFL EQIVLTRDKTVVSADLLIDDRPDITGAEPTPSWEHVLFTACHNQHLQLQP PRRRLHSWADDWKAILDSKRPC
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分子量
25 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NT5M, or cytosolic 5'-nucleotidase, is an enzyme implicated in nucleotide metabolism, playing a crucial role in the dephosphorylation of nucleotides, particularly in the regulation of cellular nucleotide pools. Given its significant function in purine and pyrimidine metabolism, NT5M has emerged as a focal point in various biological and clinical research areas, including cancer biology, cellular signaling, and metabolic disorders. Abnormal expression or activity of NT5M has been associated with several pathological conditions, suggesting its potential as a biomarker or therapeutic target. Recent studies have utilized recombinant NT5M proteins to investigate its enzymatic properties, substrate specificity, and role in cellular signaling pathways. Moreover, understanding the structural dynamics of NT5M through reconstitution and characterization of recombinant forms has provided insights into its regulatory mechanisms and interactions with other cellular components. Such research is pivotal for elucidating the biochemical mechanisms underlying cellular metabolism and for the development of targeted therapies in diseases where nucleotide metabolism is dysregulated.
