Analytical Data
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Gene name
NT5C3B
- Application
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Alternative Names
NT5C3B;NT5C3L;7-methylguanosine phosphate-specific 5'-nucleotidase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q969T7
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Expression Region
1-300aa
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AA Sequence
MAEEVSTLMK ATVLMRQPGR VQEIVGALRK GGGDRLQVIS DFDMTLSRFA YNGKRCPSSY NILDNSKIIS EECRKELTAL LHHYYPIEID PHRTVKEKLP HMVEWWTKAH NLLCQQKIQK FQIAQVVRES NAMLREGYKT FFNTLYHNNI PLFIFSAGIG DILEEIIRQM KVFHPNIHIV SNYMDFNEDG FLQGFKGQLI HTYNKNSSAC ENSGYFQQLE GKTNVILLGD SIGDLTMADG VPGVQNILKI GFLNDKVEER RERYMDSYDI VLEKDETLDV VNGLLQHILC QGVQLEMQGP
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NT5C3B, or Cytosolic 5' Nucleotidase 3B, is a protein encoded by the NT5C3B gene, which plays a critical role in nucleotide metabolism, particularly in the dephosphorylation of nucleotides and nucleosides. This enzyme is crucial for maintaining the balance of nucleotide pools within cells, contributing to various physiological processes, including DNA and RNA synthesis and cellular signaling. Dysregulation of NT5C3B has been implicated in several diseases, including cancer and neurological disorders, where altered nucleotide metabolism can affect cell proliferation and apoptosis. The recombinant expression of NT5C3B in research settings aims to better understand its structure-function relationships and regulatory mechanisms. Researchers have expressed this protein in various systems, such as bacterial or mammalian cells, allowing for detailed biochemical analysis and screening of potential inhibitors or modulators. The study of NT5C3B not only enhances our understanding of nucleotide metabolism but also opens avenues for developing targeted therapies for diseases associated with its dysregulation. Additionally, the elucidation of NT5C3B's role in enzymatic pathways may provide insights into novel therapeutic strategies that can exploit its function or dysfunction in pathological conditions. As research progresses, NT5C3B remains a significant focus, with potential implications for drug development and therapeutic interventions.
