Analytical Data
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基因名
NT5C2
- Application
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别名
NT5C2;NT5B;NT5CP;PNT5;Cytosolic purine 5'-nucleotidase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49902
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表达区间
1-561aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E
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分子量
67 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NT5C2 (5'-nucleotidase, cytosolic 2) is an important enzyme involved in nucleotide metabolism, playing a crucial role in the regulation of intracellular nucleotide levels. Its primary function is to catalyze the dephosphorylation of nucleotides, converting ATP (adenosine triphosphate) and ADP (adenosine diphosphate) into AMP (adenosine monophosphate), which is vital for various cellular processes, including energy homeostasis and signal transduction. Dysregulation of NT5C2 has been implicated in several pathological conditions, including cancer, where it may contribute to tumor progressions, such as promoting cell proliferation and suppressing apoptosis. Additionally, mutations and overexpression of NT5C2 have been associated with hematological malignancies, particularly acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML), making it a target for therapeutic intervention. Research on NT5C2, including the study of its recombinant protein, aims to elucidate its structure, regulation, and functional role in cellular metabolism. Understanding the mechanisms by which NT5C2 operates can provide insights into potential therapeutic strategies for conditions related to nucleotide dysregulation, emphasizing its significance in both basic and translational research. The production of NT5C2 as a recombinant protein allows for detailed biochemical studies, structure-function analysis, and the exploration of its interactions with other cellular components, thereby advancing our knowledge of its role in health and disease. As researchers continue to investigate NT5C2, it holds promise as a biomarker and therapeutic target, highlighting the critical need for further exploration in both preclinical and clinical settings.
