Analytical Data
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基因名
esaB
- Application
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别名
esaB;Type VII secretion system accessory factor EsaB
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0C050
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表达区间
1-80aa
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氨基酸序列
MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISIFDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL
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分子量
16.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of esaB recombinant protein is rooted in the quest to understand bacterial pathogenesis and the immune response it evokes. EsaB, a key component of the Type III secretion system in certain pathogenic bacteria, plays a critical role in injective virulence, allowing these organisms to deliver effector proteins into host cells, thereby manipulating host cellular processes for their survival and proliferation. The manipulation of the esaB gene via recombinant DNA technology enables scientists to produce large quantities of the protein for functional studies. Researchers aim to elucidate the structure-function relationships of esaB, including its role in the secretion mechanism and interaction with host cellular components. Such studies not only advance our understanding of bacterial virulence strategies but also lay the groundwork for the development of novel therapeutic approaches. By investigating the biochemical pathways mediated by esaB, scientists seek to identify potential targets for vaccine development, contributing to the broader effort to combat infections caused by multidrug-resistant pathogens. Overall, the exploration of esaB and its recombinant protein forms is pivotal for advancing molecular microbiology and infectious disease research.












