Analytical Data
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Gene name
NANS
- Application
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Alternative Names
NANS;SAS;Sialic acid synthase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NR45
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Expression Region
1-359aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMPLELELCPGRWVGGQHPCFIIAEIGQNHQ GDLDVAKRMIRMAKECGADCAKFQKSELEFKFNRKALERPYTSKHSWGKT YGEHKRHLEFSHDQYRELQRYAEEVGIFFTASGMDEMAVEFLHELNVPFF KVGSGDTNNFPYLEKTAKKGRPMVISSGMQSMDTMKQVYQIVKPLNPNFC FLQCTSAYPLQPEDVNLRVISEYQKLFPDIPIGYSGHETGIAISVAAVAL GAKVLERHITLDKTWKGSDHSASLEPGELAELVRSVRLVERALGSPTKQL LPCEMACNEKLGKSVVAKVKIPEGTILTMDMLTVKVGEPKGYPPEDIFNL VGKKVLVTVEEDDTIMEELVDNHGKKIKS
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Molecular Weight
42 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NANS (N-acetylneuraminic acid synthase) is an important enzyme involved in the biosynthesis of sialic acid, a crucial component of glycoconjugates on the cell surface that play significant roles in cell signaling, immunity, and pathogen recognition. The interest in the study of NANS recombinant protein has grown due to its potential applications in biotechnology and medicine. Understanding NANS function can enhance our knowledge of sialylation processes, which are vital for proper physiological functions and have implications in various diseases, including cancer, neurodegenerative disorders, and infectious diseases. Recombinant NANS protein production allows researchers to investigate the enzyme's structural properties, substrate specificity, and catalytic mechanisms in detail. Additionally, exploring engineered variants of NANS can yield insights into its potential biotechnological applications, such as the development of novel therapeutics and diagnostic tools targeting sialic acid pathways. The increasing prevalence of sialylation-related diseases emphasizes the need for deeper research into NANS, and by utilizing recombinant technologies, researchers aim to unravel the complexities of sialic acid metabolism, leading to advancements in both fundamental science and applied biotechnology.
