Analytical Data
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Gene name
xylF
- Application
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Alternative Names
xylF；xylT；D-xylose-binding periplasmic Protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P37387
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Expression Region
24-330aa
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AA Sequence
KEVKIGM AIDDLRLERW QKDRDIFVKK AESLGAKVFV QSANGNEETQ MSQIENMINR GVDVLVIIPY NGQVLSNVVK EAKQEGIKVL AYDRMINDAD IDFYISFDNE KVGELQAKAL VDIVPQGNYF LMGGSPVDNN AKLFRAGQMK VLKPYVDSGK IKVVGDQWVD GWLPENALKI MENALTANNN KIDAVVASND ATAGGAIQAL SAQGLSGKVA ISGQDADLAG IKRIAAGTQT MTVYKPITLL ANTAAEIAVE LGNGQEPKAD TTLNNGLKDV PSRLLTPIDV NKNNIKDTVI KDGFHKESEL
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Molecular Weight
35.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the xylF gene, which encodes for a key enzyme involved in the degradation of xylenes, is significant in the context of bioremediation and environmental biotechnology. Xylenes, commonly found as pollutants in soil and groundwater due to industrial activities, pose environmental and health risks. The xylF gene is part of the xylene degradation pathway in certain bacteria, such as Pseudomonas species, facilitating the conversion of toxic xylenes into less harmful compounds. Research on the recombinant protein produced by the xylF gene aims to elucidate the enzyme's structure, function, and catalytic mechanisms. By expressing the xylF gene in a suitable host, scientists can obtain large amounts of the enzyme for detailed biochemical studies. Understanding the properties of this recombinant protein not only enhances the fundamental knowledge of microbial metabolism but also informs strategies for developing microbial strains or enzymes that can be applied to efficiently bioremediate contaminated sites, thereby contributing to environmental conservation efforts. Ultimately, the insights gained from xylF protein studies can lead to innovative solutions for managing xylene pollution and improving the efficacy of bioremediation processes.
