Analytical Data
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Gene name
AMPK Alpha 1
- Application
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Alternative Names
AMPK Alpha 1；5'-AMP-activated Protein kinase subunit gamma-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13131
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Expression Region
1-559aa
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AA Sequence
MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG HKVAVKILNR QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI FMVMEYVSGG ELFDYICKNG RLDEKESRRL FQQILSGVDY CHRHMVVHRD LKPENVLLDA HMNAKIADFG LSNMMSDGEF LRTSCGSPNY AAPEVISGRL YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD FYLATSPPDS FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ GVRKAKWHLG IRSQSRPNDI MAEVCRAIKQ LDYEWKVVNP YYLRVRRKNP VTSTYSKMSL QLYQVDSRTY LLDFRSIDDE ITEAKSGTAT PQRSGSVSNY RSCQRSDSDA EAQGKSSEVS LTSSVTSLDS SPVDLTPRPG SHTIEFFEMC ANLIKILAQ
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Molecular Weight
64 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AMP-activated protein kinase (AMPK) is a crucial energy sensor in cells, playing a vital role in maintaining cellular energy homeostasis. The AMPK heterotrimeric complex consists of a catalytic alpha (α) subunit and regulatory beta (β) and gamma (γ) subunits. Among the two isoforms of the alpha subunit, AMPK alpha 1 (AMPKα1) has garnered significant attention due to its involvement in various metabolic processes, including glucose uptake, fatty acid oxidation, and the regulation of mitochondrial biogenesis. Research has shown that AMPKα1 is activated in response to increased levels of AMP, facilitating the switch from anabolic to catabolic pathways, particularly during stress conditions such as exercise or nutrient deprivation. Furthermore, AMPKα1 has been associated with the pathophysiology of several diseases, including obesity, diabetes, and cancer, highlighting its potential as a therapeutic target. The exploration of AMPKα1 recombinant protein is crucial for elucidating its role in cellular metabolism and signaling pathways. By studying the structure, function, and regulatory mechanisms of AMPKα1, researchers aim to develop new strategies for modulating its activity, thereby influencing metabolic health and addressing metabolic disorders. The production of AMPKα1 recombinant protein allows for detailed biochemical and biophysical analyses, paving the way for potential therapeutic interventions that leverage the AMPK pathway to restore metabolic balance and improve overall health outcomes.
