Analytical Data
-
基因名
pyrB
- Application
-
别名
pyrB；Aspartate carbamoyltransferase catalytic subunit
-
种属
E.coli
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
B7MLQ3
-
表达区间
1-311aa
-
氨基酸序列
MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL
-
分子量
41.9 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PyrB, a crucial protein involved in the regulation of pyrimidine biosynthesis, has garnered considerable attention in recent years due to its role in cellular metabolism and potential applications in synthetic biology. PyrB acts as a sensor that modulates gene expression in response to intracellular pyrimidine levels, thereby ensuring homeostasis within the cell. This regulation is particularly important in microbial systems, where the balance of nucleotide pools can significantly impact growth and productivity. The understanding of PyrB's function and mechanisms has implications for bioengineering, especially in the development of microbial strains optimized for the production of biopharmaceuticals, biofuels, and other valuable metabolites. Recent advances in recombinant DNA technology have enabled the enhancement of PyrB expression, allowing researchers to study its structure, function, and interactions with other cellular components in detail. Furthermore, the ability to manipulate PyrB in various microbial hosts opens new avenues for biotechnological applications, including the design of strains with improved metabolic pathways for enhanced product yields. Overall, the study of PyrB and its recombinant forms not only enriches our understanding of microbial metabolism but also paves the way for innovative strategies in metabolic engineering and synthetic biology.
