Analytical Data
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Gene name
pyrB
- Application
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Alternative Names
pyrB；Aspartate carbamoyltransferase catalytic subunit
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
B7MLQ3
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Expression Region
1-311aa
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AA Sequence
MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL
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Molecular Weight
41.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PyrB, a crucial protein involved in the regulation of pyrimidine biosynthesis, has garnered considerable attention in recent years due to its role in cellular metabolism and potential applications in synthetic biology. PyrB acts as a sensor that modulates gene expression in response to intracellular pyrimidine levels, thereby ensuring homeostasis within the cell. This regulation is particularly important in microbial systems, where the balance of nucleotide pools can significantly impact growth and productivity. The understanding of PyrB's function and mechanisms has implications for bioengineering, especially in the development of microbial strains optimized for the production of biopharmaceuticals, biofuels, and other valuable metabolites. Recent advances in recombinant DNA technology have enabled the enhancement of PyrB expression, allowing researchers to study its structure, function, and interactions with other cellular components in detail. Furthermore, the ability to manipulate PyrB in various microbial hosts opens new avenues for biotechnological applications, including the design of strains with improved metabolic pathways for enhanced product yields. Overall, the study of PyrB and its recombinant forms not only enriches our understanding of microbial metabolism but also paves the way for innovative strategies in metabolic engineering and synthetic biology.
