Analytical Data
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基因名
PLCz1
- Application
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别名
PLCz1；1-phosphatidylinositol 4.5-bisphosphate phosphodiesterase zeta-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q86YW0
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表达区间
322-572aa
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氨基酸序列
KETGVKKLPGVMLFKKKKTRKLKIALALSDLVIYTKAEKFKSFQHSRLYQQFNENNSIGETQARKLSKLRVHEFIFHTRKFITRIYPKATRADSSNFNPQEFWNIGCQMVALNFQTPGLPMDLQNGKFLDNGGSGYILKPHFLRESKSYFNPSNIKEGMPITLTIRLISGIQLPLTHSSSNKGDSLVIIEVFGVPNDQMKQQTRVIKKNAFSPRWNETFTFIIHVPELALIRFVVEGQGLIAGNEFLGQYT
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分子量
29 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLCz1, or phospholipase C zeta 1, is a pivotal enzyme that plays a crucial role in the regulation of intracellular calcium signaling, particularly during the process of oocyte fertilization and early embryonic development. Initially identified in mammalian sperm, PLCz1 is responsible for generating inositol trisphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4,5-bisphosphate (PIP2), leading to the release of calcium ions from the endoplasmic reticulum. This calcium signal is essential for initiating various cellular processes, including egg activation, cell cycle progression, and embryo development. Abnormalities in PLCz1 function have been linked to infertility and developmental disorders, highlighting its significance in reproductive biology and developmental genetics. The study of recombinant PLCz1 proteins allows researchers to dissect the molecular mechanisms underlying its enzymatic activity, interactions with other proteins, and its role in calcium signaling pathways. Understanding PLCz1 further is crucial for developing potential therapeutic strategies for infertility treatments and elucidating the fundamental processes of cellular signaling and development. The ongoing research aims to provide insight into its structure-function relationship, regulatory mechanisms, and impacts of mutations, thereby contributing to a deeper comprehension of its biological significance.
