Analytical Data
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基因名
PLOD1
- Application
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别名
PLOD1；LLH；PLOD；Procollagen-lysine.2-oxoglutarate 5-dioxygenase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q02809
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表达区间
1-727aa
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氨基酸序列
MRPLLLLALLGWLLLAEAKGDAKPEDNLLVLTVATKETEGFRRFKRSAQFFNYKIQALGLGEDWNVEKGTSAGGGQKVRLLKKALEKHADKEDLVILFADSYDVLFASGPRELLKKFRQARSQVVFSAEELIYPDRRLETKYPVVSDGKRFLGSGGFIGYAPNLSKLVAEWEGQDSDSDQLFYTKIFLDPEKREQINITLDHRCRIFQNLDGALDEVVLKFEMGHVRARNLAYDTLPVLIHGNGPTKLQLNYLGNYIPRFWTFETGCTVCDEGLRSLKGIGDEALPTVLVGVFIEQPTPFVSLFFQRLLRLHYPQKHMRLFIHNHEQHHKAQVEEFLAQHGSEYQSVKLVGPEVRMANADARNMGADLCRQDRSCTYYFSVDADVALTEPNSLRLLIQQNKNVIAPLMTRHGRLWSNFWGALSADGYYARSEDYVDIVQGRRVGVWNVPYISNIYLIKGSALRGELQSSDLFHHSKLDPDMAFCANIRQQDVFMFLTNRHTLGHLLSLDSYRTTHLHNDLWEVFSNPEDWKEKYIHQNYTKALAGKLVETPCPDVYWFPIFTEVACDELVEEMEHFGQWSLGNNKDNRIQGGYENVPTIDIHMNQIGFEREWHKFLLEYIAPMTEKLYPGYYTRAQFDLAFVVRYKPDEQPSLMPHHDASTFTINIALNRVGVDYEGGGCRFLRYNCSIRAPRKGWTLMHPGRLTHYHEGLPTTRGTRYIAVSFVDP
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分子量
83.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLOD1 (Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 1) encodes an enzyme that plays a critical role in collagen synthesis by catalyzing the hydroxylation of lysine residues in collagen precursors. This post-translational modification is essential for the stability and integrity of collagen fibers, which are crucial for maintaining the structural integrity of various tissues. Abnormal functioning of PLOD1 has been linked to several connective tissue disorders, including osteogenesis imperfecta and scleroderma. Recent studies have highlighted the potential of PLOD1 as a target for therapeutic interventions aimed at enhancing collagen formation and repairing tissue damage. Furthermore, with the growing interest in regenerative medicine and tissue engineering, PLOD1 recombinant protein is being explored for its ability to improve the mechanical properties of engineered tissues. By producing PLOD1 recombinantly, researchers can better understand its functional mechanisms, evaluate its role in disease pathology, and assess its suitability for developing novel therapies. Ongoing investigations into the structure-function relationship of PLOD1, alongside advances in protein engineering techniques, are expected to shed light on its potential applications in both basic research and clinical settings, paving the way for innovative strategies to treat collagen-related disorders and tissue degeneration.
