Analytical Data
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基因名
MEP1b
- Application
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别名
MEP1B;Meprin A subunit beta
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q16820
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表达区间
1-652aa
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氨基酸序列
MDLWNLSWFL FLDALLVISG LATPENFDVD GGMDQDIFDI NEGLGLDLFE GDIRLDRAQI RNSIIGEKYR WPHTIPYVLE DSLEMNAKGV ILNAFERYRL KTCIDFKPWA GETNYISVFK GSGCWSSVGN RRVGKQELSI GANCDRIATV QHEFLHALGF WHEQSRSDRD DYVRIMWDRI LSGREHNFNT YSDDISDSLN VPYDYTSVMH YSKTAFQNGT EPTIVTRISD FEDVIGQRMD FSDSDLLKLN QLYNCSSSLS FMDSCSFELE NVCGMIQSSG DNADWQRVSQ VPRGPESDHS NMGQCQGSGF FMHFDSSSVN VGATAVLESR TLYPKRGFQC LQFYLYNSGS ESDQLNIYIR EYSADNVDGN LTLVEEIKEI PTGSWQLYHV TLKVTKKFRV VFEGRKGSGA SLGGLSIDDI NLSETRCPHH IWHIRNFTQF IGSPNGTLYS PPFYSSKGYA FQIYLNLAHV TNAGIYFHLI SGANDDQLQW PCPWQQATMT LLDQNPDIRQ RMSNQRSITT DPFMTTDNGN YFWDRPSKVG TVALFSNGTQ FRRGGGYGTS AFITHERLKS RDFIKGDDVY ILLTVEDISH LNSTQIQLTP APSVQDLCSK TTCKNDGVCT VRDGKAECRC QSGEDWWYMG ERCEKRGSTR DT
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分子量
73 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MEP1b, or Methylthio-adenosine phosphorylase 1b, is a key enzyme involved in the methionine salvage pathway, which recycles byproducts of methionine metabolism. This enzyme has garnered interest due to its potential implications in various biological processes, including cellular stress responses, cancer metabolism, and viral infections. Research indicates that studying MEP1b can provide insights into metabolic regulation and its implications in disorders like cancer, where altered metabolism is a hallmark. Furthermore, MEP1b has been linked to the virulence of certain pathogens, making it a target for therapeutic interventions. By examining the structure and function of recombinant MEP1b, researchers aim to elucidate its role in cellular metabolism and explore its potential as a biomarker or therapeutic target in diseases characterized by dysfunctional methionine metabolism. Additionally, understanding its enzymatic properties can help in developing strategies to manipulate its activity for therapeutic purposes. This research is particularly relevant in the context of metabolic engineering and synthetic biology, where MEP1b could be harnessed to optimize biotechnological applications, such as the production of valuable metabolites. Overall, the study of MEP1b encompasses a multidisciplinary approach, combining biochemistry, molecular biology, and therapeutic research to unravel its complexities in human health and disease.
