Analytical Data
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Gene name
bepA
- Application
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Alternative Names
bepA；yfgC；Beta-barrel assembly-enhancing protease
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P66948
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Expression Region
28-487aa
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AA Sequence
DSADTLPDMGTSAGSTLSIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINNDEINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLTWVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFDPQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYLAKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQPLLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQPQEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLLSSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM
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Molecular Weight
58.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BepA, a type III secretion effector protein, has garnered significant interest in the field of microbiology and host-pathogen interactions, particularly concerning its role in bacterial virulence and immune evasion. This protein is secreted by various pathogenic bacteria, such as Burkholderia pseudomallei and Burkholderia mallei, which are known to cause severe diseases like melioidosis and glanders. The study of BepA is crucial because it is involved in modulating host cell processes, promoting bacterial survival within host tissues, and facilitating infection through the manipulation of host signaling pathways. Given the rising global health concerns regarding antibiotic resistance and the serious nature of infections caused by these bacteria, understanding the molecular mechanisms of BepA's action can provide insights into potential therapeutic targets. Researchers employ various recombinant DNA techniques to produce BepA in laboratory settings, allowing for in-depth studies on its structure, function, and interaction with host cells. This research not only enhances our understanding of bacterial pathogenesis but also contributes to the development of novel vaccines and treatment strategies, highlighting the importance of BepA in infectious disease research.
