Analytical Data
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基因名
MDH1
- Application
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别名
MDH1;MDHA;Malate dehydrogenase. cytoplasmic
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P40925
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表达区间
1-334aa
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氨基酸序列
MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVL DGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKD LLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKE NFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKV KLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAI CDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFV EGLPINDFSREKMDLTAKELTEEKESAFEFLSSALEHHHHHH
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分子量
37 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MDH1 (Malate Dehydrogenase 1) is an important enzyme involved in the tricarboxylic acid (TCA) cycle, playing a crucial role in cellular metabolism by catalyzing the conversion of malate to oxaloacetate while reducing NAD+ to NADH. Given its essential function in energy production and metabolic regulation, MDH1 has attracted considerable research interest in various fields, including cancer biology, metabolic disorders, and cellular signaling. Abnormal expression or function of MDH1 has been linked to several pathologies, highlighting its potential as a therapeutic target. Recent advances in molecular biology techniques, such as recombinant protein expression and purification, have enabled the production of MDH1 in heterologous systems, facilitating detailed studies of its structure and function. By analyzing the biochemical properties and interactions of MDH1, researchers aim to uncover its role in metabolic pathways and pathophysiological conditions. Furthermore, investigating MDH1’s potential as a biomarker for disease progression and as a target for drug development is critically important in ongoing biomedical research. Consequently, the recombinant expression of MDH1 not only enhances our understanding of its physiological roles but also paves the way for innovative therapeutic strategies.
