Analytical Data
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Gene name
MCM7
- Application
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Alternative Names
MCM7;CDC47;MCM2;DNA replication licensing factor MCM7
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P33993-2
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Expression Region
1-389aa
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AA Sequence
MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYV DLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLD VYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSSKPRVIR EVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTF MPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIP RSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEA HRIVKMNKSEDDESGAGELTREELRQIADVIFATVRELVSGGRSVRFSEA EQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV
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Molecular Weight
69 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MCM7, or Minichromosome Maintenance 7, is a key protein involved in DNA replication and cell cycle regulation. As a member of the MCM protein complex, it plays a crucial role in the initiation of DNA replication by unwinding the double helix, allowing for the synthesis of new DNA strands. Dysregulation of MCM7 has been implicated in various cancers, making it a significant focus of research in cancer biology. Understanding the structure and function of MCM7 is essential for elucidating its role in oncogenesis and for developing potential therapeutic strategies. Recent studies have explored the expression patterns of MCM7 in tumor tissues and its correlation with cancer progression, highlighting its potential as a biomarker for cancer diagnosis and prognosis. Additionally, investigations into MCM7's interactions with other cellular proteins and its regulation during the cell cycle have provided insights into the complex mechanisms governing cellular proliferation. The reconstitution of MCM7 as a recombinant protein allows for in-depth biochemical assays and structural studies, facilitating a better understanding of its function and interactions. This research not only contributes to our fundamental knowledge of DNA replication but also opens avenues for targeted therapies that could disrupt the function of MCM7 in cancer cells, offering hope for more effective cancer treatments.
