Analytical Data
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基因名
bbgII
- Application
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别名
bbgII;Beta-galactosidase BbgII
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
A5A2I5
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表达区间
1-689aa
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氨基酸序列
MSKRRKHSWPQPLKGAESRLWYGGDYNPDQWPEEVWDDDIRLMKKAGVNLVSVGIFSWAKIEPEEGKYDFDWLDRAIDKLGKAGIAVDLASATASPPMWLTQAHPEVLWKDERGDTVWPGAREHWRPTSPVFREYALNLCRRMAEHYKGNPYVVAWHVSNEYGCHNRFDYSDDAMRAFQKWCKKRYKTIDAVNEAWGTAFWAQHMNDFSEIIPPRYIGDGNFMNPGKLLDYKRFSSDALKELYIAERDVLESITPGLPLTTNFMVSAGGSMLDYDDWGAEVDFVSNDHYFTPGEDHFDEVAYAASLMDGISRKEPWFQMEHSTSAVNWRPINYRAEPGSVVRDSLAQVAMGADAICYFQWRQSKAGAEKWHSSMVPHAGEDSQIFRDVCELGADLGRLSDEGLMGTKTVKSKVAVVFDYESQWATEYTANPTQQVDHWTEPLDWFRALADNGITADVVPVRSDWDSYEIAVLPCVYLLSEETSRRVREFVANGGKLFVTYYTGLSDENDHIWLGGYPGSIRDVVGVRVEEFAPMGNDMPGALDHLDLDNGTVAHDFADVITSTADTSTVLASYKAERWTGMNEVPAIVANGYGDGRTVYVGCRLGRQGLAKSLPAMLGSMGLSDLAGDGRVLRVERADAAAASRFEFVFNRTHEPVTVDVEGEAIAASLAHVDDGRATIDPTGVVVLRR
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分子量
84.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BBGII (Bacillus subtilis Biofilm Glycoprotein II) is a significant protein involved in biofilm formation in Bacillus subtilis, a model organism for studying bacterial behavior. Research on BBGII is driven by its critical role in microbial adhesion and biofilm development, which are important for both natural ecosystems and industrial applications. Biofilms can protect bacteria from antibiotics and contribute to persistent infections, making them a major focus of study in microbiology and medicine. Understanding the structure and function of BBGII can provide insights into the molecular mechanisms of biofilm formation, potentially leading to novel strategies for preventing biofilm-related infections and improving bioprocesses in industrial biotechnology. Additionally, as biofilms are relevant in various environments, from natural water systems to medical devices and manufacturing, the study of BBGII's interactions with other molecules could lead to broader applications in controlling microbial populations. Consequently, the characterization and manipulation of BBGII through recombinant protein technology not only enhance the understanding of biofilm dynamics but also pave the way for innovative approaches to manage biofilms in diverse settings.












