Analytical Data
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基因名
MAT2A
- Application
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别名
MAT2A;AMS2;MATA2;S-adenosylmethionine synthase isoform type-2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P31153
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表达区间
1-395aa
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氨基酸序列
MNGQLNGFHEAFIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY
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分子量
45.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MAT2A (methionine adenosyltransferase 2A) is a critical enzyme involved in the synthesis of S-adenosylmethionine (SAM), a pivotal methyl donor in various biological methylation processes. Research into MAT2A has garnered attention due to its significant role in cellular metabolism, particularly in regulating the levels of methionine and SAM, which are essential for numerous biochemical reactions, including DNA methylation and the synthesis of neurotransmitters. Dysregulation of MAT2A has been implicated in various diseases, including cancer, neurological disorders, and liver diseases, where abnormal methylation patterns can lead to altered gene expression and cellular function. The reconstitution of MAT2A as a recombinant protein enables detailed studies of its enzymatic activity, structural properties, and regulatory mechanisms. This research is critical for understanding how MAT2A contributes to metabolic homeostasis and the potential therapeutic implications of targeting its pathways in disease contexts. By characterizing MAT2A through recombinant techniques, scientists aim to develop strategies for modulation of its activity, which could pave the way for novel interventions in diseases characterized by methionine and SAM metabolism imbalances.
