Analytical Data
-
Gene name
MAT2A
- Application
-
Alternative Names
MAT2A;AMS2;MATA2;S-adenosylmethionine synthase isoform type-2
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P31153
-
Expression Region
1-395aa
-
AA Sequence
MNGQLNGFHEAFIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY
-
Molecular Weight
45.2 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MAT2A (methionine adenosyltransferase 2A) is a critical enzyme involved in the synthesis of S-adenosylmethionine (SAM), a pivotal methyl donor in various biological methylation processes. Research into MAT2A has garnered attention due to its significant role in cellular metabolism, particularly in regulating the levels of methionine and SAM, which are essential for numerous biochemical reactions, including DNA methylation and the synthesis of neurotransmitters. Dysregulation of MAT2A has been implicated in various diseases, including cancer, neurological disorders, and liver diseases, where abnormal methylation patterns can lead to altered gene expression and cellular function. The reconstitution of MAT2A as a recombinant protein enables detailed studies of its enzymatic activity, structural properties, and regulatory mechanisms. This research is critical for understanding how MAT2A contributes to metabolic homeostasis and the potential therapeutic implications of targeting its pathways in disease contexts. By characterizing MAT2A through recombinant techniques, scientists aim to develop strategies for modulation of its activity, which could pave the way for novel interventions in diseases characterized by methionine and SAM metabolism imbalances.
