Analytical Data
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Gene name
KLHL14
- Application
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Alternative Names
KLHL14; KIAA1384Kelch-like protein 14; Protein interactor of Torsin-1A; Printor; Protein interactor of torsinA
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9P2G3
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Expression Region
1-628aa
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AA Sequence
MSRSGDRTSTFDPSHSDNLLHGLNLLWRKQLFCDVTLTAQGQQFHCHKAVLASCSQYFRSLFSSHPPLGGGVGGQDGLGAPKDQQQPPQQQPSQQQQPPPQEEPGTPSSSPDDKLLTSPRAINNLVLQGCSSIGLRLVLEYLYTANVTLSLDTVEEVLSVSKILHIPQVTKLCVQFLNDQISVQNYKQVCKIAALHGLEETKKLANKYLVEDVLLLNFEEMRALLDSLPPPVESELALFQMSVLWLEHDRETRMQYAPDLMKRLRFALIPAPELVERVQSVDFMRTDPVCQKLLLDAMNYHLMPFRQHCRQSLASRIRSNKKMLLLVGGLPPGPDRLPSNLVQYYDDEKKTWKILTIMPYNSAHHCVVEVENFLFVLGGEDQWNPNGKHSTNFVSRYDPRFNSWIQLPPMQERRASFYACRLDKHLYVIGGRNETGYLSSVECYNLETNEWRYVSSLPQPLAAHAGAVHNGKIYISGGVHNGEYVPWLYCYDPVMDVWARKQDMNTKRAIHTLAVMNDRLYAIGGNHLKGFSHLDVMLVECYDPKGDQWNILQTPILEGRSGPGCAVLDDSIYLVGGYSWSMGAYKSSTICYCPEKGTWTELEGDVAEPLAGPACVTVILPSCVPYNK
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Molecular Weight
97.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KLHL14, a member of the Kelch-like protein family, has garnered significant interest in recent years due to its potential roles in various biological processes, including cellular signaling, protein degradation, and cytoskeletal organization. Mutations in the KLHL14 gene have been implicated in several diseases, notably in certain forms of intellectual disabilities and developmental disorders, emphasizing its crucial function in neuronal development and function. The protein is known to interact with Cullin-RING ubiquitin ligase complexes, facilitating the ubiquitination process, which is essential for proteasomal degradation of specific substrates. Given the dysregulation of protein homeostasis in many pathologies, including cancer and neurodegenerative diseases, understanding the biochemistry and functionality of KLHL14 is of paramount importance. Recent studies have focused on the recombinant expression of KLHL14 proteins to elucidate their structural and functional properties. By employing various expression systems, researchers aim to obtain sufficient quantities of pure KLHL14 protein for detailed biochemical assays and structural analyses. This work is expected to provide insights into the molecular mechanisms by which KLHL14 modulates cellular activities and its potential as a therapeutic target. Advances in this area may lead to novel strategies for intervening in diseases where KLHL14 is implicated, paving the way for future research into targeted therapies that can restore normal functions associated with this essential protein.
