Analytical Data
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基因名
KLF8
- Application
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别名
Basic krueppel-like factor 3; BKLF3; DXS741; KLF8; KLF8_HUMAN; Krueppel-like factor 8; Kruppel like factor 8; MGC138314; Zinc finger protein 741; ZNF741
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95600
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表达区间
1-359aa
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氨基酸序列
MVDMDKLINNLEVQLNSEGGSMQVFKQVTASVRNRDPPEIEYRSNMTSPTLLDANPMENPALFNDIKIEPPEELLASDFSLPQVEPVDLSFHKPKAPLQPASMLQAPIRPPKPQSSPQTLVVSTSTSDMSTSANIPTVLTPGSVLTSSQSTGSQQILHVIHTIPSVSLPNKMGGLKTIPVVVQSLPMVYTTLPADGGPAAITVPLIGGDGKNAGSVKVDPTSMSPLEIPSDSEESTIESGSSALQSLQGLQQEPAAMAQMQGEESLDLKRRRIHQCDFAGCSKVYTKSSHLKAHRRIHTGEKPYKCTWDGCSWKFARSDELTRHFRKHTGIKPFRCTDCNRSFSRSDHLSLHRRRHDTM
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分子量
65.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KLF8, a member of the Krüppel-like factor (KLF) family of transcription factors, has garnered significant attention in recent years due to its pivotal role in regulating cellular processes such as proliferation, differentiation, and apoptosis. The KLF family is implicated in various biological functions, and KLF8, in particular, is known to be involved in inflammatory responses, embryonic development, and tumorigenesis. Aberrant expression of KLF8 has been associated with several human cancers, suggesting that it may act as an oncogene. Research has shown that KLF8 can enhance cell invasion and metastasis, making it a potential target for therapeutic intervention. To better understand KLF8's mechanisms of action, scientists have sought to produce recombinant KLF8 protein, enabling investigations into its structural features, DNA-binding properties, and interaction with other proteins. This research is crucial for elucidating the pathways through which KLF8 exerts its effects, thereby providing insights into its role in cancer biology and potential implications for targeted therapies. High-level production of recombinant KLF8, utilizing techniques such as bacterial or mammalian expression systems, allows for extensive biochemical and biophysical analyses that can shed light on its functions and therapeutic potential. Overall, the study of recombinant KLF8 protein is expected to advance our knowledge of this critical regulator and its contributions to disease processes, ultimately leading to novel strategies for cancer diagnosis and treatment.
