Analytical Data
-
Gene name
HIST1H2AD
- Application
-
Alternative Names
HIST1H2AD；H2AFG；HIST1H2AD；Histone H2A type 1-D
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P20671
-
Expression Region
2-130aa
-
AA Sequence
SGRGKQGGK ARAKAKTRSS RAGLQFPVGR VHRLLRKGNY SERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGK VTIAQGGVLP NIQAVLLPKK TESHHKAKGK
-
Molecular Weight
14.1 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HIST1H2AD is a histone variant that plays a crucial role in chromatin structure and gene regulation. It is part of the histone H2A family and is particularly significant in the context of epigenetics, where it influences the accessibility of DNA for transcription and the overall orchestration of cellular processes. The study of HIST1H2AD has gained traction due to its implications in various biological processes, including development, differentiation, and response to environmental cues. Notably, aberrations in histone variants like HIST1H2AD have been linked to several diseases, including cancer, where changes in the histone code can lead to altered gene expression patterns and tumorigenesis. Researchers are utilizing reconstitution methods to produce recombinant HIST1H2AD proteins, allowing for in-depth studies of its biochemical properties and interactions with other proteins and nucleic acids. By understanding the structure and function of HIST1H2AD, researchers aim to elucidate its role in chromatin dynamics and its potential as a therapeutic target in diseases associated with histone modifications. This investigation is essential for advancing our knowledge of epigenetic regulation and developing strategies for medical intervention in disorders linked to histone alterations.
