Analytical Data
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Gene name
RNF168
- Application
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Alternative Names
RNF168；E3 ubiquitin-Protein ligase RNF168
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IYW5
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Expression Region
1-571aa
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AA Sequence
MALPKDAIPSLSECQCGICMEILVEPVTLPCNHTLCKPCFQSTVEKASLCCPFCRRRVSSWTRYHTRRNSLVNVELWTIIQKHYPRECKLRASGQESEEVADDYQPVRLLSKPGELRREYEEEISKVAAERRASEEEENKASEEYIQRLLAEEEEEEKRQAEKRRRAMEEQLKSDEELARKLSIDINNFCEGSISASPLNSRKSDPVTPKSEKKSKNKQRNTGDIQKYLTPKSQFGSASHSEAVQEVRKDSVSKDIDSSDRKSPTGQDTEIEDMPTLSPQISLGVGEQGADSSIESPMPWLCACGAEWYHEGNVKTRPSNHGKELCVLSHERPKTRVPYSKETAVMPCGRTESGCAPTSGVTQTNGNNTGETENEESCLLISKEISKRKNQESSFEAVKDPCFSAKRRKVSPESSPDQEETEINFTQKLIDLEHLLFERHKQEEQDRLLALQLQKEVDKEQMVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKDGNFKRQTHTKHPTPERGSRDKNRQVSLKMQLKQSVNRRKMPNSTRDHCKVSKSAHSLQPSISQKSVFQMFQRCTK
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Molecular Weight
66.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF168 is an E3 ubiquitin ligase that plays a crucial role in the DNA damage response, particularly in the context of double-strand break (DSB) repair. Its involvement in the regulation of the ubiquitination of key proteins at sites of DNA lesions makes it a vital component of the cellular mechanism that maintains genomic integrity. RNF168 is known to facilitate the recruitment of other repair factors, such as 53BP1 and BRCA1, by promoting a polyubiquitination signal that is critical for initiating downstream repair pathways. Aberrations in the RNF168 function can lead to enhanced sensitivity to DNA-damaging agents and may contribute to the development of various cancers. Consequently, studying RNF168 not only enhances our understanding of DNA repair mechanisms but also opens potential avenues for therapeutic interventions targeting DNA damage response pathways. Additionally, the recombinant expression of RNF168 allows for in-depth biochemical analyses and structural studies, providing insights into its functional domains and the molecular interactions that underpin its role in the DNA damage response. As researchers continue to explore the intricacies of RNF168's functions, it holds promise for elucidating novel regulatory pathways and finding new strategies for cancer treatment.
