Analytical Data
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Gene name
KRT8
- Application
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Alternative Names
KRT38;HHA8;HKA8;KRTHA8;Keratin. type I cuticular Ha8
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P05787
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Expression Region
1-483aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSMSIRVTQKSYKVSTSGPRAFSSRSYTS GPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLV LEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQ QQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKN KYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQL YEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAE AESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQ RASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQEL MNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAY GGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSES SDVLPK
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Molecular Weight
56 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Keratin 8 (KRT8) is a crucial intermediate filament protein predominantly expressed in simple epithelial cells and plays a significant role in maintaining cell structure and function. Research on KRT8 has gained momentum due to its involvement in various cellular processes, including apoptosis, differentiation, and response to stress. Abnormal expression of KRT8 has been linked to several pathological conditions, including liver diseases, cancers, and other forms of epithelial dysfunction. Studies have shown that KRT8 can act as a marker for certain cancer types, offering potential diagnostic and therapeutic avenues. Additionally, the understanding of KRT8's role in cellular signaling and tissue integrity has highlighted its importance in developmental biology and regenerative medicine. The recombinant expression of KRT8 in different systems allows for detailed analysis of its functional properties and interactions with other proteins, facilitating a deeper understanding of its biological roles. This research is essential for developing targeted therapies and improving clinical outcomes for diseases associated with keratin abnormalities.











