Analytical Data
-
Gene name
KCNE4
- Application
-
Alternative Names
KCNE4; Potassium voltage-gated channel subfamily E member 4; MinK-related peptide 3; Minimum potassium ion channel-related peptide 3; Potassium channel subunit beta MiRP3
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q8WWG9
-
Expression Region
1-170aa
-
AA Sequence
MLKMEPLNSTHPGTAASSSPLESRAAGGGSGNGNEYFYILVVMSFYGIFLIGIMLGYMKSKRREKKSSLLLLYKDEERLWGEAMKPLPVVSGLRSVQVPLMLNMLQESVAPALSCTLCSMEGDSVSSESSSPDVHLTIQEEGADEELEETSETPLNESSEGSSENIHQNS
-
Molecular Weight
44.44 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KCNE4, a member of the KCNE family of potassium channel auxiliary subunits, plays a significant role in modulating the activity of voltage-gated potassium channels. It is primarily expressed in tissues such as the heart, brain, and lungs, where it influences key physiological functions, including cardiac repolarization and neuronal excitability. Research into KCNE4 is important because altered expression or dysfunction of this protein has been linked to various cardiac arrhythmias and other pathophysiological conditions. Furthermore, KCNE4 interacts with different potassium channel alpha subunits, particularly KCNQ1 and KCNQ4, impacting their current amplitude and gating properties. This regulatory role highlights the significance of KCNE4 in maintaining proper cellular excitability and contributes to our understanding of potassium channelopathies. Recent studies employing recombinant KCNE4 proteins have provided insights into its structure-function relationships, elucidating how specific amino acid residues influence its interactions with alpha subunits. These findings have implications for the development of targeted therapies for diseases associated with potassium channel dysregulation. Continued exploration of KCNE4 dynamics and its biological consequences is crucial for advancing our knowledge of cardiac and neuronal health, as well as potential therapeutic interventions in related disorders.
